Decker, J and Weinberger, K and Prohaska, E and Hauck, S and Kosslinger, C and Wolf, H and Hengerer, A (2000) Characterization of a human pancreatic secretory trypsin inhibitor mutant binding to Legionella pneumophila as determined by a quartz crystal microbalance. JOURNAL OF IMMUNOLOGICAL METHODS, 233 (1-2). pp. 159-165. ISSN 0022-1759,
Full text not available from this repository.Abstract
We describe the isolation from a large phagemid library of a human pancreatic secretory trypsin inhibitor (hPSTI) mutant that binds to Legionella pneumophila. To gain further insight into the binding kinetics of the isolated hPSTI mutant, an immunosensing system based on a quartz crystal microbalance (QCM) was used. In contrast to ELISA procedures, k(on) and k(off) rates could be derived from the QCM sensograms. Thus, it is possible to characterize specific intermolecular interactions between proteins and phages isolated from large phage display libraries by QCM. (C) 2000 Elsevier Science B.V. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | MEMBRANE-PROTEINS; PHAGE ANTIBODIES; VARIANTS; DISPLAY; HPSTI; affinity constant; human pancreatic secretory trypsin inhibitor; immunosensor; phage display; quartz crystal microbalance |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Medizinische Mikrobiologie und Hygiene |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 18 May 2021 12:03 |
| Last Modified: | 18 May 2021 12:03 |
| URI: | https://pred.uni-regensburg.de/id/eprint/42901 |
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