Baumann, O and Arlt, K and Rommling, K and Goller, H and Walz, B (2000) Characterization of an extremely motile cellular network in the rotifer Asplanchna spp. - Structure, kinetics, and the cytoskeleton. CELL AND TISSUE RESEARCH, 299 (1). pp. 159-172. ISSN 0302-766X, 1432-0878
Full text not available from this repository. (Request a copy)Abstract
The pseudocoelomic body cavity of the rotifer Asplanchna spp. contains free cells that form a highly dynamic, three-dimensional polygonal network of filopodia. Using video-enhanced differential interference contrast microscopy, we have qualitatively and quantitatively characterized the motion types involved with network motility: (1) filopodial junctions are displaced laterally at 10.52+/-0.46 mu m/s; (2) free-ending filopodia form and extend at rates of 8.77+/-0.40 mu m/s, until they retract again at 7.23+/-0.87 mu m/s; (3) filopodial strands fuse either laterally or tip to the lateral side. The combination of these motion types results in enlargements, diminutions, and extinctions of filopodial polygons, and in the formation of new polygons. Moreover, there is intense and fast (5.11+/-0.28 mu m/s) particle transport within the filopodial strands. The organization of the cytoskeleton in filopodia was examined by electron microscopy and by labeling with fluorescent-tagged phalloidin. Filopodia contain several microtubules that are often organized in a bundle. Moreover, F-actin is present within the filopodia. To characterize which of these cytoskeletal systems is involved with cell and organelle motility, we have examined cell dynamics after incubations with colchicine or cytochalasin D. The results of these pharmacological experiments provide evidence that microtubules are required for both cell and organelle motility, but that actin filaments contribute to these phenomena and are required for the structural maintenance of slender filopodia.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ORGANELLE TRANSPORT; ACTIN-FILAMENTS; ALLOGROMIA-LATICOLLARIS; RETICULOPODIAL NETWORK; MICROTUBULES INVITRO; DYNAMIC INSTABILITY; FLEXURAL RIGIDITY; MOVEMENT; MYOSIN; RETICULOMYXA; cell motility; intracellular transport; cytoskeleton; microtubules; actin filaments; Asplanchna spp. (Rotifera) |
| Subjects: | 500 Science > 590 Zoological sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Zoologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 18 May 2021 06:41 |
| Last Modified: | 18 May 2021 06:41 |
| URI: | https://pred.uni-regensburg.de/id/eprint/42933 |
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