Peltomaa, Riikka and Farka, Zdenek and Mickert, Matthias J. and Brandmeier, Julian C. and Pastucha, Matej and Hlavacek, Antonin and Martinez-Orts, Monica and Canales, Angeles and Skladal, Petr and Benito-Pena, Elena and Moreno-Bondi, Maria C. and Gorris, Hans H. (2020) Competitive upconversion-linked immunoassay using peptide mimetics for the detection of the mycotoxin zearalenone. BIOSENSORS & BIOELECTRONICS, 170: 112683. ISSN 0956-5663, 1873-4235
Full text not available from this repository. (Request a copy)Abstract
Due to increasing food safety standards, the analysis of mycotoxins has become essential in the food industry. In this work, we have developed a competitive upconversion-linked immunosorbent assay (ULISA) for the analysis of zearalenone (ZEA), one of the most frequently encountered mycotoxins in food worldwide. Instead of a toxin conjugate conventionally used in competitive immunoassays, we designed a ZEA mimicking peptide extended by a biotin-linker and confirmed its excellent suitability to mimic ZEA by nuclear magnetic resonance (NMR) and surface plasmon resonance (SPR) analysis. Upconversion nanoparticles (UCNP, type NaYF4:Yb,Tm) served as background-free optical label for the detection of the peptide mimetic in the competitive ULISA. Streptavidinconjugated UCNPs were prepared by click reaction using an alkyne-PEG-neridronate linker. The UCNP conjugate clearly outperformed conventional labels such as enzymes or fluorescent dyes. With a limit of detection of 20 pg mL-1 (63 pM), the competitive ULISA is well applicable to the detection of ZEA at the levels set by the European legislation. Moreover, the ULISA is specific for ZEA and its metabolites (alpha and beta-zearalenol) without significant cross-reactivity with other related mycotoxins. We detected ZEA in spiked and naturally contaminated maize samples using liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) as a reference method to demonstrate food analysis in real samples.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ULTRASENSITIVE DETECTION; FUMONISIN B-1; NANOPARTICLES; REGULATIONS; MIMOTOPE; GOLD; Zearalenone; Upconversion nanoparticle; Peptide mimetic; Immunosensing; Surface plasmon resonance; Food safety |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 05 Mar 2021 07:45 |
| Last Modified: | 05 Mar 2021 07:45 |
| URI: | https://pred.uni-regensburg.de/id/eprint/43130 |
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