Koschnitzki, Dagmar and Moeller, Ralf and Leuko, Stefan and Przybyla, Bartos and Beblo-Vranesevic, Kristina and Wirth, Reinhard and Huber, Harald and Rachel, Reinhard and Rettberg, Petra (2021) Questioning the radiation limits of life: Ignicoccus hospitalis between replication and VBNC. ARCHIVES OF MICROBIOLOGY, 203. pp. 1299-1308. ISSN 0302-8933, 1432-072X
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Radiation of ionizing or non-ionizing nature has harmful effects on cellular components like DNA as radiation can compromise its proper integrity. To cope with damages caused by external stimuli including radiation, within living cells, several fast and efficient repair mechanisms have evolved. Previous studies addressing organismic radiation tolerance have shown that radiotolerance is a predominant property among extremophilic microorganisms including (hyper-) thermophilic archaea. The analysis of the ionizing radiation tolerance of the chemolithoautotrophic, obligate anaerobic, hyperthermophilic Crenarchaeon Ignicoccus hospitalis showed a D-10-value of 4.7 kGy, fourfold exceeding the doses previously determined for other extremophilic archaea. The genome integrity of I. hospitalis after gamma-ray exposure in relation to its survival was visualized by RAPD and qPCR. Furthermore, the discrimination between reproduction, and ongoing metabolic activity was possible for the first time indicating that a potential viable but non-culturable (VBNC) state may also account for I. hospitalis.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | CELL-CYCLE CHARACTERISTICS; MITOCHONDRIAL-DNA DAMAGE; IONIZING-RADIATION; ESCHERICHIA-COLI; SP-NOV; REPAIR; RESISTANCE; SURVIVAL; ARCHAEA; GAMMA; Ignicoccus hospitalis; Radiation; Survival; Genome integrity; Extremophiles; VBNC |
| Subjects: | 500 Science > 560 Paleontology Paleozoology 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Mikrobiologie (Archaeenzentrum) > Prof. Dr. Reinhard Wirth Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Mikrobiologie (Archaeenzentrum) > Prof. Dr. Reinhard Rachel |
| Depositing User: | Petra Gürster |
| Date Deposited: | 21 Apr 2021 13:52 |
| Last Modified: | 21 Apr 2021 13:52 |
| URI: | https://pred.uni-regensburg.de/id/eprint/43132 |
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