Wunderlich, Lucia C. S. and Strohl, Florian and Strohl, Stefan and Vanderpoorten, Oliver and Mascheroni, Luca and Kaminski, Clemens F. (2021) Superresolving the kidney-a practical comparison of fluorescence nanoscopy of the glomerular filtration barrier. ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 413 (4). pp. 1203-1214. ISSN 1618-2642, 1618-2650
Full text not available from this repository. (Request a copy)Abstract
Immunofluorescence microscopy is routinely used in the diagnosis of and research on renal impairments. However, this highly specific technique is restricted in its maximum resolution to about 250 nm in the lateral and 700 nm in the axial directions and thus not sufficient to investigate the fine subcellular structure of the kidney's glomerular filtration barrier. In contrast, electron microscopy offers high resolution, but this comes at the cost of poor preservation of immunogenic epitopes and antibody penetration alongside a low throughput. Many of these drawbacks were overcome with the advent of super-resolution microscopy methods. So far, four different super-resolution approaches have been used to study the kidney: single-molecule localization microscopy (SMLM), stimulated emission depletion (STED) microscopy, structured illumination microscopy (SIM), and expansion microscopy (ExM), however, using different preservation methods and widely varying labelling strategies. In this work, all four methods were applied and critically compared on kidney slices obtained from samples treated with the most commonly used preservation technique: fixation by formalin and embedding in paraffin (FFPE). Strengths and weaknesses, as well as the practicalities of each method, are discussed to enable users of super-resolution microscopy in renal research make an informed decision on the best choice of technique. The methods discussed enable the efficient investigation of biopsies stored in kidney banks around the world.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | STRUCTURED ILLUMINATION MICROSCOPY; STIMULATED-EMISSION; ELECTRON-MICROSCOPY; SLIT DIAPHRAGM; IMMUNOFLUORESCENCE; AUTOFLUORESCENCE; MECHANISMS; NEPHRIN; PROTEIN; RECONSTRUCTION; Kidney; Nephrin; Podocyte foot processes; Super-resolution microscopy; Fluorescence microscopy; Formalin-fixed paraffin-embedded |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Abteilung für Nephrologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 17 May 2021 15:39 |
| Last Modified: | 17 May 2021 15:39 |
| URI: | https://pred.uni-regensburg.de/id/eprint/43169 |
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