Mellert, Kevin and Benckendorff, Julian and Leithaeuser, Frank and Zimmermann, Katarzyna and Wiegand, Peter and Frascaroli, Giada and Buck, Michaela and Malaise, Muriel and Hartmann, Gunther and Barchet, Winfried and Fuerst, Daniel and Mytilineos, Joannis and Mayer-Steinacker, Regine and Viardot, Andreas and Moeller, Peter (2020) U-DCS: characterization of the first permanent human dendritic sarcoma cell line. SCIENTIFIC REPORTS, 10 (1): 21221. ISSN 2045-2322,
Full text not available from this repository. (Request a copy)Abstract
A dendritic cell sarcoma cell line, U-DCS, was established from a dendritic cell sarcoma in a 53-year-old Caucasian male patient. Since its establishment, U-DCS has maintained stable phenotypic characteristics in vitro and has a doubling time of approximately 2 days under standard culture conditions. U-DCS is growing with typical dendritic cell morphology in tissue and expresses the dendritic cell sarcoma immunophenotypic markers S100 protein, MHCI, MHCII, and vimentin. Expression analysis revealed transcripts for the toll-like receptors TLR3, -4, -9 and DDX58 (RIG-I), but not for TLR2. U-DCS shows functional features of dendritic cells with the ability of phagocytosis and antigen-specific T cell stimulation. Karyotype-, CGH-, and mFISH analysis point to a chromosomal instability and a hypotetraploid karyotype with approximately 130 chromosomes. U-DCS is the first immortalized human dendritic cell sarcoma cell line and has some morphological and functional features of dendritic cells without dependency on growth factors.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | LEUKEMIA/SMALL LYMPHOCYTIC LYMPHOMA; INTERDIGITATING RETICULUM-CELL; ANTIGEN-PRESENTING CELLS; PATHOGEN RECOGNITION; LANGERHANS CELL; B-CELLS; SUBSETS; BLOOD; DIFFERENTIATION; INTERLEUKIN-8; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Abteilung für Pädiatrische Hämatologie, Onkologie und Stammzelltransplantation |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 05 Mar 2021 08:56 |
| Last Modified: | 05 Mar 2021 08:56 |
| URI: | https://pred.uni-regensburg.de/id/eprint/43173 |
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