Blawat, Kim and Mayr, Alexandra and Hardt, Miriam and Kirschneck, Christian and Nokhbehsaim, Marjan and Behl, Christian and Deschner, James and Jaeger, Andreas and Memmert, Svenja (2020) Regulation of Autophagic Signaling by Mechanical Loading and Inflammation in Human PDL Fibroblasts. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 21 (24): 9446. ISSN , 1422-0067
Full text not available from this repository. (Request a copy)Abstract
Autophagy (cellular self-consumption) is a crucial adaptation mechanism during cellular stress conditions. This study aimed to examine how this important process is regulated in human periodontal ligament (PDL) fibroblasts by mechanical and inflammatory stress conditions and whether the mammalian target of rapamycin (mTOR) signaling pathway is involved. Autophagy was quantified by flow cytometry. Qualitative protein phosphorylation profiling of the mTOR pathway was carried out. Effects of mTOR regulation were assessed by quantification of important synthesis product collagen 1, cell proliferation and cell death with real-time PCR and flow cytometry. Autophagy as a response to mechanical or inflammatory treatment in PDL fibroblasts was dose and time dependent. In general, autophagy was induced by stress stimulation. Phosphorylation analysis of mTOR showed regulatory influences of mechanical and inflammatory stimulation on crucial target proteins. Regulation of mTOR was also detectable via changes in protein synthesis and cell proliferation. Physiological pressure had cell-protective effects (p = 0.025), whereas overload increased cell death (p = 0.003), which was also promoted in long-term inflammatory treatment (p < 0.001). Our data provide novel insights about autophagy regulation by mechanical and inflammatory stress conditions in human PDL fibroblasts. Our results suggest some involvement of the mTOR pathway in autophagy and cell fate regulation under the named conditions.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | PERIODONTAL-LIGAMENT CELLS; RECEPTOR ACTIVATOR; STRESS; MTOR; PHOSPHORYLATION; DIFFERENTIATION; OSTEOBLASTS; EXPRESSION; PHYSIOLOGY; IL-1-BETA; autophagy; mammalian target of rapamycin (mTOR) signaling pathway; inflammatory conditions; mechanical stress; orthodontic tooth movement |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Kieferorthopädie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 08 Mar 2021 06:39 |
| Last Modified: | 08 Mar 2021 06:39 |
| URI: | https://pred.uni-regensburg.de/id/eprint/43241 |
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