Lage-Rupprecht, Vanessa and Zhou, Li and Bianchini, Gaia and Aghvami, S. Sara and Mueller, Max and Rozsa, Balazs and Sassoe-Pognetto, Marco and Egger, Veronica (2020) Presynaptic NMDARs cooperate with local spikes toward GABA release from the reciprocal olfactory bulb granule cell spine. ELIFE, 9: e63737. ISSN 2050-084X,
Full text not available from this repository. (Request a copy)Abstract
In the rodent olfactory bulb the smooth dendrites of the principal glutamatergic mitral cells (MCs) form reciprocal dendrodendritic synapses with large spines on GABAergic granule cells (GC), where unitary release of glutamate can trigger postsynaptic local activation of voltage-gated Na+-channels (Navs), that is a spine spike. Can such single MC input evoke reciprocal release? We find that unitary-like activation via two-photon uncaging of glutamate causes GC spines to release GABA both synchronously and asynchronously onto MC dendrites. This release indeed requires activation of Navs and high-voltage-activated Ca2+-channels (HVACCs), but also of NMDA receptors (NMDAR). Simulations show temporally overlapping HVACC- and NMDAR-mediated Ca2+-currents during the spine spike, and ultrastructural data prove NMDAR presence within the GABAergic presynapse. This cooperative action of presynaptic NMDARs allows to implement synapse-specific, activity-dependent lateral inhibition, and thus could provide an efficient solution to combinatorial percept synthesis in a sensory system with many receptor channels.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | EXTERNAL PLEXIFORM LAYER; LONG-LASTING DEPOLARIZATIONS; LATERAL INHIBITION; MITRAL CELLS; DENDRODENDRITIC SYNAPSES; PARVALBUMIN INTERNEURONS; GLUTAMATE RECEPTORS; MITRAL/TUFTED CELLS; GABAERGIC NEURONS; DENDRITIC SPINES; |
| Subjects: | 500 Science > 570 Life sciences 500 Science > 590 Zoological sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Zoologie Biology, Preclinical Medicine > Institut für Zoologie > Neurophysiologie (Prof. Dr. Veronica Egger) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 08 Mar 2021 07:49 |
| Last Modified: | 08 Mar 2021 07:49 |
| URI: | https://pred.uni-regensburg.de/id/eprint/43321 |
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