Mustroph, Julian and Hupf, Julian and Hanses, Frank and Evert, Katja and Baier, Maria J. and Evert, Matthias and Meindl, Christine and Wagner, Stefan and Hubauer, Ute and Pietrzyk, Gabriela and Leininger, Simon and Staudner, Stephan and Vogel, Manuel and Wallner, Stefan and Zimmermann, Markus and Sossalla, Samuel and Maier, Lars S. and Jungbauer, Carsten (2021) Decreased GLUT1/NHE1 RNA expression in whole blood predicts disease severity in patients with COVID-19. ESC HEART FAILURE, 8 (1). pp. 309-316. ISSN 2055-5822
Full text not available from this repository. (Request a copy)Abstract
Aims We aimed to assess whether expression of whole-blood RNA of sodium proton exchanger 1 (NHE1) and glucose transporter 1 (GLUT1) is associated with COVID-19 infection and outcome in patients presenting to the emergency department with respiratory infections. Furthermore, we investigated NHE1 and GLUT1 expression in the myocardium of deceased COVID-19 patients. Methods and results Whole-blood quantitative assessment of NHE1 and GLUT1 RNA was performed using quantitative PCR in patients with respiratory infection upon first contact in the emergency department and subsequently stratified by SARS-CoV-2 infection status. Assessment of NHE1 and GLUT1 RNA using PCR was also performed in left ventricular myocardium of deceased COVID-19 patients. NHE1 expression is up-regulated in whole blood of patients with COVID-19 compared with other respiratory infections at first medical contact in the emergency department (control: 0.0021 +/- 0.0002, COVID-19: 0.0031 +/- 0.0003, P = 0.01). The ratio of GLUT1 to NHE1 is significantly decreased in the blood of COVID-19 patients who are subsequently intubated and/or die (severe disease) compared with patients with moderate disease (moderate disease: 0.497 +/- 0.083 vs. severe disease: 0.294 +/- 0.0336, P = 0.036). This ratio is even further decreased in the myocardium of patients who deceased from COVID-19 in comparison with the myocardium of non-infected donors. Conclusions NHE1 and GLUT1 may be critically involved in the disease progression of SARS-CoV-2 infection. We show here that SARS-CoV-2 infection critically disturbs ion channel expression in the heart. A decreased ratio of GLUT1/NHE1 could potentially serve as a biomarker for disease severity in patients with COVID-19.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | CARDIAC NA+/H+ EXCHANGER; GLUCOSE TRANSPORTERS; GENE-EXPRESSION; DOWN-REGULATION; HEART; EMPAGLIFLOZIN; INHIBITION; NHE1; HYPERTROPHY; ACTIVATION; COVID-19; Infection; Heart; GLUT1; NHE1 |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin I Medicine > Lehrstuhl für Innere Medizin II Medicine > Lehrstuhl für Klinische Chemie und Laboratoriumsmedizin Medicine > Lehrstuhl für Pathologie |
| Depositing User: | Petra Gürster |
| Date Deposited: | 21 Apr 2021 08:10 |
| Last Modified: | 21 Apr 2021 08:10 |
| URI: | https://pred.uni-regensburg.de/id/eprint/43346 |
Actions (login required)
 |
View Item |
