Guendel, Fabian and Kofoed-Branzk, Michael and Gronke, Konrad and Tizian, Caroline and Witkowski, Mario and Cheng, Hung-Wei and Heinz, Gitta Anne and Heinrich, Frederik and Durek, Pawel and Norris, Paula S. and Ware, Carl F. and Ruedl, Christiane and Herold, Susanne and Pfeffer, Klaus and Hehlgans, Thomas and Waisman, Ari and Becher, Burkhard and Giannou, Anastasios D. and Brachs, Sebastian and Ebert, Karolina and Tanriver, Yakup and Ludewig, Burkhard and Mashreghi, Mir-Farzin and Kruglov, Andrey A. and Diefenbach, Andreas (2020) Group 3 Innate Lymphoid Cells Program a Distinct Subset of IL-22BP-Producing Dendritic Cells Demarcating Solitary Intestinal Lymphoid Tissues. IMMUNITY, 53 (5). 1015-+. ISSN 1074-7613, 1097-4180
Full text not available from this repository. (Request a copy)Abstract
Solitary intestinal lymphoid tissues such as cryptopatches (CPs) and isolated lymphoid follicles (ILFs) constitute steady-state activation hubs containing group 3 innate lymphoid cells (ILC3) that continuously produce interleukin (IL)-22. The outer surface of CPs and ILFs is demarcated by a poorly characterized population of CD11c(+) cells, Using genome-wide single-cell transcriptional profiling of intestinal mononuclear phagocytes and multidimensional flow cytometry, we found that CP- and ILF-associated CD11c(+) cells were a transcriptionally distinct subset of intestinal cDCs, which we term CIA-DCs. CIA-DCs required programming by CP- and ILF-resident CCR6+ ILC3 via lymphotoxin-beta receptor signaling in cDCs. CIA-DCs differentially expressed genes associated with immunoregulation and were the major cellular source of IL-22 binding protein (IL-22BP) at steady state. Mice lacking CIA-DC-derived IL-22BP exhibited diminished expression of epithelial lipid transporters, reduced lipid resorption, and changes in body fat homeostasis. Our findings provide insight into the design principles of an immunoregulatory checkpoint controlling nutrient absorption.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ROR-GAMMA-T; GREEN FLUORESCENT PROTEIN; SIGNALING CONTROLS; INDUCER CELLS; RECEPTOR; LYMPHOTOXIN; EXPRESSION; IL-22; MICE; MACROPHAGES; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Immunologie Medicine > Zentren des Universitätsklinikums Regensburg > Regensburger Centrum für Interventionelle Immunologie (RCI) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 08 Mar 2021 07:57 |
| Last Modified: | 08 Mar 2021 07:57 |
| URI: | https://pred.uni-regensburg.de/id/eprint/43356 |
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