Graf, Johannes and Kretz, Markus (2020) From structure to function: Route to understanding lncRNA mechanism. BIOESSAYS, 42 (12): e202000027. ISSN 0265-9247, 1521-1878
Full text not available from this repository. (Request a copy)Abstract
RNAs have emerged as a major target for diagnostics and therapeutics approaches. Regulatory nonprotein-coding RNAs (ncRNAs) in particular display remarkable versatility. They can fold into complex structures and interact with proteins, DNA, and other RNAs, thus modulating activity, localization, or interactome of multi-protein complexes. Thus, ncRNAs confer regulatory plasticity and represent a new layer of regulatory control. Interestingly, long noncoding RNAs (lncRNAs) tend to acquire complex secondary and tertiary structures and their function-in many cases-is dependent on structural conservation rather than primary sequence conservation. Whereas for many proteins, structure and its associated function are closely connected, for lncRNAs, the structural domains that determine functionality and its interactome are still not well understood. Numerous approaches for analyzing the structural configuration of lncRNAs have been developed recently. Here, will provide an overview of major experimental approaches used in the field, and discuss the potential benefit of using combinatorial strategies to analyze lncRNA modes of action based on structural information.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | RNA SECONDARY STRUCTURE; LONG NONCODING RNA; X-CHROMOSOME INACTIVATION; STRUCTURE PREDICTION; PRIMER EXTENSION; SHAPE; REVEALS; PROTEIN; TRANSCRIPTOME; PURIFICATION; lncRNA; long noncoding RNA |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Biochemie I |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 08 Mar 2021 08:43 |
| Last Modified: | 08 Mar 2021 08:43 |
| URI: | https://pred.uni-regensburg.de/id/eprint/43396 |
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