Seica, Ana Filipa Santos and Iancu, Cristina V. and Pfeilschifter, Benedikt and Madej, M. Gregor and Choe, Jun-Yong and Hellwig, Petra (2020) Asp(22) drives the protonation state of the Staphylococcus epidermidis glucose/H+ symporter. JOURNAL OF BIOLOGICAL CHEMISTRY, 295 (45). pp. 15253-15261. ISSN 0021-9258, 1083-351X
Full text not available from this repository. (Request a copy)Abstract
The Staphylococcus epidermidis glucose/H+ symporter (GlcP(Se)) is a membrane transporter highly specific for glucose and a homolog of the human glucose transporters (GLUT, SLC2 family). Most GLUTs and their bacterial counterparts differ in the transport mechanism, adopting uniport and sugar/H+ symport, respectively. Unlike other bacterial GLUT homologs (for example, XylE), GlcP(Se) has a loose H+/sugar coupling. Asp(22) is part of the proton-binding site of GlcP(Se) and crucial for the glucose/H+ co-transport mechanism. To determine how pH variations affect the proton site and the transporter, we performed surface-enhanced IR absorption spectroscopy on the immobilized GlcP(Se). We found that Asp(22) has a pK(a) of 8.5 +/- 0.1, a value consistent with that determined previously for glucose transport, confirming the central role of this residue for the transport mechanism of GlcP(Se). A neutral replacement of the negatively charged Asp(22) led to positive charge displacements over the entire pH range, suggesting that the polarity change of the WT reflects the protonation state of Asp(22). We expected that the substitution of the residue Ile(105) for a serine, located within hydrogen-bonding distance to Asp(22), would change the microenvironment, but the pK(a) of Asp(22) corresponded to that of the WT. A167E mutation, selected in analogy to the XylE, introduced an additional protonatable site and perturbed the protonation state of Asp(22), with the latter now exhibiting a pK(a) of 6.4. These studies confirm that Asp(22) is the proton-binding residue in GlcP(Se) and show that charged residues in its vicinity affect the pK(a) of glucose/H+ symport.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ESCHERICHIA-COLI; INFRARED-SPECTROSCOPY; HYDROPHOBIC INTERIOR; CRYSTAL-STRUCTURE; LACTOSE PERMEASE; PK(A) VALUES; AMINO-ACIDS; MECHANISM; TRANSPORTERS; PROTEIN; transport proteins; membrane proteins; proton transport; surface-enhanced infrared spectroscopy; major facilitator superfamily; transport; membrane transport; spectroscopy; glucose transport; major facilitator family; pK value |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biophysik und physikalische Biochemie > Prof. Dr. Christine Ziegler |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 08 Mar 2021 09:33 |
| Last Modified: | 08 Mar 2021 09:33 |
| URI: | https://pred.uni-regensburg.de/id/eprint/43401 |
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