Weider, Matthias and Schroeder, Agnes and Docheva, Denitsa and Rodrian, Gabriele and Enderle, Isabel and Seidel, Corinna Lesley and Andreev, Darja and Wegner, Michael and Bozec, Aline and Deschner, James and Kirschneck, Christian and Proff, Peter and Goelz, Lina (2020) A Human Periodontal Ligament Fibroblast Cell Line as a New Model to Study Periodontal Stress. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 21 (21): 7961. ISSN , 1422-0067
Full text not available from this repository. (Request a copy)Abstract
The periodontal ligament (PDL) is exposed to different kinds of mechanical stresses such as bite force or orthodontic tooth movement. A simple and efficient model to study molecular responses to mechanical stress is the application of compressive force onto primary human periodontal ligament fibroblasts via glass disks. Yet, this model suffers from the need for primary cells from human donors which have a limited proliferative capacity. Here we show that an immortalized cell line, PDL-hTERT, derived from primary human periodontal ligament fibroblasts exhibits characteristic responses to glass disk-mediated compressive force resembling those of primary cells. These responses include induction and secretion of pro-inflammatory markers, changes in expression of extracellular matrix-reorganizing genes and induction of genes related to angiogenesis, osteoblastogenesis and osteoclastogenesis. The fact that PDL-hTERT cells can easily be transfected broadens their usefulness, as molecular gain- and loss-of-function studies become feasible.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ORTHODONTIC TOOTH MOVEMENT; TISSUE; OSTEOCLASTOGENESIS; UNIQUE; mechanical loading; orthodontic tooth movement; bone remodeling; cell culture techniques |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Unfallchirurgie Medicine > Lehrstuhl für Kieferorthopädie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 08 Mar 2021 10:41 |
| Last Modified: | 08 Mar 2021 10:41 |
| URI: | https://pred.uni-regensburg.de/id/eprint/43471 |
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