Puetz, P. and Behrent, A. and Baeumner, Antje J. and Wegener, Joachim (2020) Laser-scribed graphene (LSG) as new electrode material for impedance-based cellular assays. SENSORS AND ACTUATORS B-CHEMICAL, 321: 128443. ISSN , 0925-4005
Full text not available from this repository. (Request a copy)Abstract
Impedance-based monitoring of cell-based assays has evolved to a multi-functional tool in fundamental and applied biomedical research. The majority of studies rely on gold-films as the electrode material which serves as growth surface and electrode at a time. Besides all its favorable properties like inertness, biocompatibility and superior electrochemical characteristics, gold-film electrodes in contact to cell culture medium show a capacitance of the electrode/electrolyte interface that may become limiting for the sensitivity of the readout. This study describes the use of laser-scribed graphene (LSG) as an alternative electrode material in impedance-based cell monitoring. LSG electrodes are prepared from commercial polyimide foils by simple CO2-laser-induced carbonization. The resulting electrodes show a 25times larger interface capacitance than standard gold-film electrodes due to their foam-like surface topography. Furthermore, LSG-electrodes are (i) highly compatible with cell attachment, (ii) easy to prepare in customized geometries from mu m to cm with tunable surface topography and (iii) accessible by roll-to-roll production lines. We conclude from time-and frequency dependent impedance measurements of cell-covered LSG electrodes in direct comparison to the reference material gold that LSG provides an enormous potential to improve the sensitivity of impedance-based monitoring and electric-field mediated manipulation of adherent cells.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | CELLS; Laser-scribed graphene; Cell-electrode junction; Cell-based assays; Cell-based sensors; Impedance spectroscopy; Electric cell-substrate impedance sensing; ECIS |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik > Chemo- und Biosensorik (Prof. Antje J. Bäumner, formerly Prof. Wolfbeis) Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik > Bioanalytik und Biosensorik (Prof. Joachim Wegener) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 09 Mar 2021 06:13 |
| Last Modified: | 09 Mar 2021 06:13 |
| URI: | https://pred.uni-regensburg.de/id/eprint/43561 |
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