Heimes, Diana and Wiesmann, Nadine and Eckrich, Jonas and Brieger, Juergen and Mattyasovszky, Stefan and Proff, Peter and Weber, Manuel and Deschner, James and Al-Nawas, Bilal and Kammerer, Peer W. (2020) In Vivo Modulation of Angiogenesis and Immune Response on a Collagen Matrix via Extracorporeal Shockwaves. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 21 (20): 7574. ISSN , 1422-0067
Full text not available from this repository. (Request a copy)Abstract
The effective management of tissue integration and immunological responses to transplants decisively co-determines the success of soft and hard tissue reconstruction. The aim of this in vivo study was to evaluate the eligibility of extracorporeal shock wave therapy (ESWT) with respect to its ability to modulate angiogenesis and immune response to a collagen matrix (CM) for tissue engineering in the chorioallantoic membrane (CAM) assay, which is performed with fertilized chicken eggs. CM were placed on the CAM on embryonic development day (EDD) 7; at EDD-10, ESWT was conducted at 0.12 mJ/mm(2) with 500 impulses each. One and four days later, angiogenesis represented by vascularized area, vessel density, and vessel junctions as well as HIF-1 alpha and VEGF gene expression were evaluated. Furthermore, immune response (iNOS2, MMP-9, and MMP-13 via qPCR) was assessed and compared between ESWT- and non-ESWT-groups. At EDD-14, the vascularized area (+115% vs. +26%) and the increase in vessel junctions (+751% vs. +363%) were significantly higher in the ESWT-group. ESWT significantly increased MMP-9 gene expression at EDD-11 and significantly decreased MMP-13 gene expression at EDD-14 as compared to the controls. Using the CAM assay, an enhanced angiogenesis and neovascularization in CM after ESWT were observed. Furthermore, ESWT could reduce the inflammatory activity after a latency of four days.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | TOPICAL BLOOD PERFUSION; WAVE THERAPY; MACROPHAGE PHENOTYPE; GROWTH-FACTORS; EXPRESSION; CELLS; REGENERATION; INDUCTION; MEMBRANE; MODEL; oral inflammation; angiogenesis; extracorporeal shockwave therapy; collagen matrix; mucoderm((R)); chorioallantoic membrane assay; macrophage response; vascular endothelial growth factor; matrix metalloproteases |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Kieferorthopädie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 09 Mar 2021 09:54 |
| Last Modified: | 09 Mar 2021 09:54 |
| URI: | https://pred.uni-regensburg.de/id/eprint/43639 |
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