Spirk, Marlen and Zimny, Sebastian and Neumann, Maximilian and McMullen, Nichole and Sinal, Christopher J. and Buechler, Christa (2020) Chemerin-156 is the Active Isoform in Human Hepatic Stellate Cells. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 21 (20): 7555. ISSN , 1422-0067
Full text not available from this repository. (Request a copy)Abstract
The chemokine chemerin exists as C-terminally processed isoforms whose biological functions are mostly unknown. A highly active human chemerin variant (huChem-157) was protective in experimental hepatocellular carcinoma (HCC) models. Hepatic stellate cells (HSCs) are central mediators of hepatic fibrogenesis and carcinogenesis and express the chemerin receptors chemokine-like receptor 1 (CMKLR1) and G protein-coupled receptor 1 (GPR1). Here we aimed to analyse the effect of chemerin isoforms on the viability, proliferation and secretome of the human HSC cell line LX-2. Therefore, huChem-157, 156 and 155 were over-expressed in LX-2 cells, which have low endogenous chemerin levels. HuChem-157 produced in LX-2 cells activated CMKLR1 and GPR1, and huChem-156 modestly induced GPR1 signaling. HuChem-155 is an inactive chemerin variant. Chemerin isoforms had no effect on cell viability and proliferation. Cellular expression of the fibrotic proteins galectin-3 and alpha-smooth muscle actin was not regulated by any chemerin isoform. HuChem-156 increased IL-6, IL-8 and galectin-3 in cell media. HuChem-157 was ineffective, and accordingly, did not enhance levels of these proteins in media of primary human hepatic stellate cells when added exogenously. These analyses provide evidence that huChem-156 is the biologic active chemerin variant in hepatic stellate cells and acts as a pro-inflammatory factor.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | EX-VIVO; RECEPTOR; INFLAMMATION; FIBROSIS; CMKLR1; GPR1; ACTIVATION; EXPRESSION; CHEMR23; MAST; Galectin-3; IL-6; Proliferation; Tango Assay; CMKLR1 |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin I |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 09 Mar 2021 11:48 |
| Last Modified: | 09 Mar 2021 11:48 |
| URI: | https://pred.uni-regensburg.de/id/eprint/43640 |
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