Gruening, Martina and Neuber, Sven and Nestler, Peter and Lehnfeld, Jutta and Dubs, Manuela and Fricke, Katja and Schnabelrauch, Matthias and Helm, Christiane A. and Mueller, Rainer and Staehlke, Susanne and Nebe, J. Barbara (2020) Enhancement of Intracellular Calcium Ion Mobilization by Moderately but Not Highly Positive Material Surface Charges. FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY, 8: 1016. ISSN 2296-4185,
Full text not available from this repository. (Request a copy)Abstract
Electrostatic forces at the cell interface affect the nature of cell adhesion and function; but there is still limited knowledge about the impact of positive or negative surface charges on cell-material interactions in regenerative medicine. Titanium surfaces with a variety of zeta potentials between -90 mV and +50 mV were generated by functionalizing them with amino polymers, extracellular matrix proteins/peptide motifs and polyelectrolyte multilayers. A significant enhancement of intracellular calcium mobilization was achieved on surfaces with a moderately positive (+1 to +10 mV) compared with a negative zeta potential (-90 to -3 mV). Dramatic losses of cell activity (membrane integrity, viability, proliferation, calcium mobilization) were observed on surfaces with a highly positive zeta potential (+50 mV). This systematic study indicates that cells do not prefer positive charges in general, merely moderately positive ones. The cell behavior of MG-63s could be correlated with the materials' zeta potential; but not with water contact angle or surface free energy. Our findings present new insights and provide an essential knowledge for future applications in dental and orthopedic surgery.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | CELL-CYCLE ARREST; PLASMA POLYMERIZATION; PROTEIN ADSORPTION; FUNCTIONAL-GROUPS; FOCAL ADHESION; TITANIUM; PROLIFERATION; BIOMATERIALS; CHEMISTRY; TOPOGRAPHY; human osteoblasts; calcium ion signaling; titanium surface modification; amino polymer; polyelectrolyte multilayer; zeta potential; surface charge; wettability |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Physikalische und Theoretische Chemie Chemistry and Pharmacy > Institut für Physikalische und Theoretische Chemie > Chair of Chemistry VI - Physical Chemistry (Solution Chemistry) Chemistry and Pharmacy > Institut für Physikalische und Theoretische Chemie > Chair of Chemistry VI - Physical Chemistry (Solution Chemistry) > PD Dr. Rainer Müller |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 12 Mar 2021 07:31 |
| Last Modified: | 12 Mar 2021 07:31 |
| URI: | https://pred.uni-regensburg.de/id/eprint/43825 |
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