Kataruka, Shubhangini and Modrak, Martin and Kinterova, Veronika and Malik, Radek and Zeitler, Daniela M. and Horvat, Filip and Kanka, Jiri and Meister, Gunter and Svoboda, Petr (2020) MicroRNA dilution during oocyte growth disables the microRNA pathway in mammalian oocytes. NUCLEIC ACIDS RESEARCH, 48 (14). pp. 8050-8062. ISSN 0305-1048, 1362-4962
Full text not available from this repository. (Request a copy)Abstract
MicroRNAs (miRNAs) are ubiquitous small RNAs guiding post-transcriptional gene repression in countless biological processes. However, the miRNA pathway in mouse oocytes appears inactive and dispensable for development. We propose that marginalization of the miRNA pathway activity stems from the constraints and adaptations of RNA metabolism elicited by the diluting effects of oocyte growth. We report that miRNAs do not accumulate like mRNAs during the oocyte growth because miRNA turnover has not adapted to it. The most abundant miRNAs total tens of thousands of molecules in growing (empty set 40 mu m) and fully grown (empty set 80 mu m) oocytes, a number similar to that observed in much smaller fibroblasts. The lack of miRNA accumulation results in a 100-fold lower miRNA concentration in fully grown oocytes than in somatic cells. This brings a knockdown-like effect, where diluted miRNAs engage targets but are not abundant enough for significant repression. Low-miRNA concentrations were observed in rat, hamster, porcine and bovine oocytes, arguing that miRNA inactivity is not mouse-specific but a common mammalian oocyte feature. Injection of 250,000 miRNA molecules was sufficient to restore reporter repression in mouse and porcine oocytes, suggesting that miRNA inactivity comes from low-miRNA abundance and not from some suppressor of the pathway.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | 3 ABUNDANCE CLASSES; GENE-EXPRESSION; MESSENGER-RNA; ENDOGENOUS SIRNAS; LET-7 REPRESSES; MOUSE OOCYTES; BINDING; DICER; CELL; TRANSCRIPTOME; |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Biochemie I > Prof. Dr. Gunter Meister |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 15 Mar 2021 13:35 |
| Last Modified: | 15 Mar 2021 13:35 |
| URI: | https://pred.uni-regensburg.de/id/eprint/43991 |
Actions (login required)
 |
View Item |
