Passmore, Margaret R. and Ki, Katrina K. and Chan, Chris H. H. and Lee, Talvin and Bouquet, Mahe and Wood, Emily S. and Raman, Sainath and Rozencwajg, Sacha and Burrell, Aidan J. C. and McDonald, Charles and Langguth, Daman and Shekar, Kiran and Malfertheiner, Maximilian and Fraser, John F. and Suen, Jacky Y. (2020) The effect of hyperoxia on inflammation and platelet responses in an ex vivo extracorporeal membrane oxygenation circuit. ARTIFICIAL ORGANS, 44 (12). pp. 1276-1285. ISSN 0160-564X, 1525-1594
Full text not available from this repository. (Request a copy)Abstract
Use of extracorporeal membrane oxygenation (ECMO) is expanding, however, it is still associated with significant morbidity and mortality. Activation of inflammatory and innate immune responses and hemostatic alterations contribute to complications. Hyperoxia may play a role in exacerbating these responses. Nine ex vivo ECMO circuits were tested using fresh healthy human whole blood, with two oxygen levels: 21% inspired fraction of oxygen (FiO(2); mild hyperoxia; n = 5) and 100% FiO(2)(severe hyperoxia; n = 4). Serial blood samples were taken for analysis of platelet aggregometry, leukocyte activation, inflammatory, and oxidative stress markers. ECMO resulted in reduced adenosine diphosphate- (P < .05) and thrombin receptor activating peptide-induced (P < .05) platelet aggregation, as well as increasing levels of the neutrophil activation marker, neutrophil elastase (P = .013). Additionally, levels of the inflammatory chemokine interleukin-8 were elevated (P < .05) and the activity of superoxide dismutase, a marker of oxidative stress, was increased (P = .002). Hyperoxia did not augment these responses, with no significant differences detected between mild and severe hyperoxia. Our ex vivo model of ECMO revealed that the circuit itself triggers a pro-inflammatory and oxidative stress response, however, exposure to supra-physiologic oxygen does not amplify that response. Extended-duration studies and inclusion of an endothelial component could be beneficial in characterizing longer term changes.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | INTENSIVE-CARE-UNIT; OXIDATIVE STRESS; LIFE-SUPPORT; ACTIVATION; MORTALITY; COMPLICATIONS; NEUTROPHIL; THERAPY; INJURY; DAMAGE; extracorporeal membrane oxygenation; hyperoxia; inflammation; platelets |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin II |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 16 Mar 2021 12:56 |
| Last Modified: | 16 Mar 2021 12:56 |
| URI: | https://pred.uni-regensburg.de/id/eprint/44058 |
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