Leppkes, Moritz and Knopl, Jasmin and Naschberger, Elisabeth and Lindemann, Aylin and Singh, Jeeshan and Herrmann, Irmgard and Stuerzl, Michael and Staats, Leonie and Mahajan, Aparna and Schauer, Christine and Kremer, Anita N. and Voelkl, Simon and Amann, Kerstin and Evert, Katja and Falkies, Christina and Wehrfritz, Andrea and Rieker, Ralf J. and Hartmann, Arndt and Kremer, Andreas E. and Neurath, Markus F. and Munoz, Luis E. and Schett, Georg and Herrmann, Martin (2020) Vascular occlusion by neutrophil extracellular traps in COVID-19. EBIOMEDICINE, 58: 102925. ISSN 2352-3964,
Full text not available from this repository. (Request a copy)Abstract
Background: Coronavirus induced disease 2019 (COVID-19) can be complicated by severe organ damage leading to dysfunction of the lungs and other organs. The processes that trigger organ damage in COVID-19 are incompletely understood. Methods: Samples were donated from hospitalized patients. Sera, plasma, and autopsy-derived tissue sections were examined employing flow cytometry, enzyme-linked immunosorbent assays, and immunohistochemistry. Patient findings: Here, we show that severe COVID-19 is characterized by a highly pronounced formation of neutrophil extracellular traps (NETs) inside the micro-vessels. Intravascular aggregation of NETs leads to rapid occlusion of the affected vessels, disturbed microcirculation, and organ damage. In severe COVID-19, neutrophil granulocytes are strongly activated and adopt a so-called low-density phenotype, prone to spontaneously form NETs. In accordance, markers indicating NET turnover are consistently increased in COVID-19 and linked to disease severity. Histopathology of the lungs and other organs from COVID-19 patients showed congestions of numerous micro-vessels by aggregated NETs associated with endothelial damage. Interpretation: These data suggest that organ dysfunction in severe COVID-19 is associated with excessive NET formation and vascular damage. (c) 2020 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license. (http://creativecommons.org/licenses/by-nc-nd/4.0/)
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | CORONAVIRUS; THROMBOSIS; HEPARIN; AGGREGATION; INHIBITION; PNEUMONIA; ELASTASE; HISTONES; IMMUNITY; SARS-CoV-2; Endothelialitis; Immunothrombosis; Aggregated neutrophil extracellular traps; Coagulopathy |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Pathologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 17 Mar 2021 11:56 |
| Last Modified: | 17 Mar 2021 11:56 |
| URI: | https://pred.uni-regensburg.de/id/eprint/44083 |
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