Winkler, Sebastian and Niedermair, Tanja and Fuchtmeier, Bernd and Grifka, Joachim and Graessel, Susanne and Anders, Sven and Heers, Guido and Wagner, Ferdinand (2015) The impact of hypoxia on mesenchymal progenitor cells of human skeletal tissue in the pathogenesis of heterotopic ossification. INTERNATIONAL ORTHOPAEDICS, 39 (12). pp. 2495-2501. ISSN 0341-2695, 1432-5195
Full text not available from this repository. (Request a copy)Abstract
Purpose Mesenchymal progenitor cells (MPCs) are capable of differentiating into osteo/chondrogenic cells to contribute substantially to heterotopic ossification (HO). This study aimed to examine the impact of hypoxia on MPCs in the aetiology of HO. Methods MPCs from human normal and HO skeletal tissue were cultivated under normoxia and hypoxia. Gene expression of factors which have a key role in HO aetiology (BMPs, COX-1 and COX-2, etc.) were examined by real-time PCR. Tissue of both groups was analysed by immunohistochemistry. Results Under hypoxia, COX-1, -2 and SOX-9 gene expression was elevated in HO MPCs, whereas in normal muscle tissue only COX-2 was upregulated. MPCs from HO had a significantly elevated gene expression of BMP-4 and decreased expression of BMP-1 and HIF-1 under hypoxia compared to normal MPCs. Immunohistochemistry detected no significant differences between normal and HO tissue. Conclusions Hypoxia causes an enhanced gene expression of factors, which have a key role in HO pathophysiology. A better understanding of this entity will possibly allow reducing HO rates in orthopaedic and trauma surgery.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | BONE-FORMATION; MURINE MODEL; MUSCLE; ARTHROPLASTY; CHONDROCYTES; INJURY; TRAUMA; Hypoxia; Mesenchymal progenitor cell; Heterotopic ossification; Pathogenesis |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Orthopädie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 07 May 2019 08:15 |
| Last Modified: | 07 May 2019 08:15 |
| URI: | https://pred.uni-regensburg.de/id/eprint/4416 |
Actions (login required)
 |
View Item |
