Antosch, Martin and Schubert, Veit and Holzinger, Philipp and Houben, Andreas and Grasser, Klaus D. (2015) Mitotic lifecycle of chromosomal 3xHMG-box proteins and the role of their N-terminal domain in the association with rDNA loci and proteolysis. NEW PHYTOLOGIST, 208 (4). pp. 1067-1077. ISSN 0028-646X, 1469-8137
Full text not available from this repository. (Request a copy)Abstract
The high mobility group (HMG)-box is a DNA-binding domain characteristic of various eukaryotic DNA- binding proteins. 3xHMG-box proteins (containing three copies of the HMG-box domain and a unique basic N-terminal domain) are specific for plants and the Arabidopsis genome encodes two versions termed 3xHMG-box1 and 3xHMG-box2, whose expression is cell cycle-dependent, peaking during mitosis. Here, we analysed in detail the spatiotemporal expression, subcellular localisation and chromosome association of the Arabidopsis thaliana 3xHMG-box proteins. Live cell imaging and structured illumination microscopy revealed that the expression of the 3xHMG-box proteins is induced in late G2 phase of the cell cycle and upon nuclear envelope breakdown in prophase they rapidly associate with the chromosomes. 3xHMG-box1 associates preferentially with 45S rDNA loci and the basic N-terminal domain is involved in the targeting of rDNA loci. Shortly after mitosis the 3xHMG-box proteins are degraded and an N-terminal destruction-box mediates the proteolysis. Ectopic expression/localisation of 3xHMG-box1 in interphase nuclei results in reduced plant growth and various developmental defects including early bolting and abnormal flower morphology. The remarkable conservation of 3xHMG-box proteins within the plant kingdom, their characteristic expression during mitosis, and their striking association with chromosomes, suggest that they play a role in the organisation of plant mitotic chromosomes.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | DNA-BINDING PROTEINS; MOBILITY GROUP PROTEINS; CELL-CYCLE; HMG-BOX; ARABIDOPSIS-THALIANA; LINKER HISTONES; CHROMATIN; PLANTS; INTERACTS; FAMILY; Arabidopsis; chromatin; high mobility group (HMG)-box domain; HMGB; mitotic chromosome; rDNA; selective proteolysis |
| Subjects: | 500 Science > 580 Botanical sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Pflanzenwissenschaften > Lehrstuhl für Zellbiologie und Pflanzenphysiologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 06 May 2019 07:27 |
| Last Modified: | 06 May 2019 07:27 |
| URI: | https://pred.uni-regensburg.de/id/eprint/4419 |
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