Arndt, Stephanie and Lissner, Clara and Unger, Petra and Baeumler, Wolfgang and Berneburg, Mark and Karrer, Sigrid (2020) Biological effects of a new ultraviolet A(1)prototype based on light-emitting diodes on the treatment of localized scleroderma. EXPERIMENTAL DERMATOLOGY, 29 (12). pp. 1199-1208. ISSN 0906-6705, 1600-0625
Full text not available from this repository. (Request a copy)Abstract
Ultraviolet A(1)(UVA(1)) phototherapy (spectral range 340-400 nm) is a well-established treatment option for various skin diseases such as localized scleroderma. Recent improvements of conventional UVA(1)light sources (metal-halide or fluorescent lamps) have brought attention to a new light-emitting diode (LED) technology with remarkable advantages in handling and clinical routine. This study provides a preclinical histological and molecular evaluation of an LED-based UVA(1)prototype with a narrower spectral range (360-400 nm) for treating localized scleroderma. Scleroderma mouse models and fibroblasts in vitro were exposed to LED-based UVA(1)phototherapy or to irradiation with a commercially available metal-halide lamp emitting low-dose (20, 40 J/cm(2)), medium-dose (60 J/cm(2)) and high-dose (80, 100 J/cm(2)) UVA(1)light. Both UVA(1)light sources affected inflammatory genes (IL-1 alpha and IL-6) and growth factors (TGFss-1 and TGFss-2). Increased collagen type 1 was reduced after UVA(1)phototherapy. Matrix metalloproteinase-1 was more enhanced after a medium dose of LED-based UVA(1)phototherapy than after conventional treatment. In vivo, dermal thickness and the amount of collagen were reduced after both treatment methods. Remarkably, myofibroblasts were more effectively reduced by a medium dose of LED-based UVA(1)phototherapy. The study indicates that LED-based UVA(1)phototherapy yields similar or even better results than conventional treatment. In terms of biosafety and patient comfort, LED-based UVA(1)phototherapy offers clear advantages over conventional treatment because of the use of a narrower and less harmful UVA(1)spectrum, less heat generation and shorter treatment times at the same irradiation intensity. Clinical studies are required to confirm these results in patients with localized scleroderma.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | GROWTH-FACTOR-BETA; HUMAN DERMAL FIBROBLASTS; BLEOMYCIN-INDUCED SCLERODERMA; UVA(1) RADIATION-THERAPY; SMOOTH MUSCLE ACTIN; A1 PHOTOTHERAPY; SINGLET OXYGEN; ANIMAL-MODEL; TGF-BETA; MATRIX METALLOPROTEINASE-1; extracellular matrix; fibroblasts; LED-based UVA(1); localized scleroderma; phototherapy |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Dermatologie und Venerologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 18 Mar 2021 07:42 |
| Last Modified: | 18 Mar 2021 07:42 |
| URI: | https://pred.uni-regensburg.de/id/eprint/44194 |
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