Schroeder, Agnes and Gubernator, Joshua and Nazet, Ute and Spanier, Gerrit and Jantsch, Jonathan and Proff, Peter and Kirschneck, Christian (2020) Effects of sodium chloride on the gene expression profile of periodontal ligament fibroblasts during tensile strain. JOURNAL OF OROFACIAL ORTHOPEDICS-FORTSCHRITTE DER KIEFERORTHOPADIE, 81 (5). pp. 360-370. ISSN 1434-5293, 1615-6714
Full text not available from this repository. (Request a copy)Abstract
Purpose During orthodontic tooth movement, pressure and tension zones develop in the periodontal ligament, and periodontal ligament fibroblasts (PDLF) become exposed to mechanical strain. Enhanced salt (NaCl) concentrations are known to modulate responses of PDLF and immune cells to different stimuli like mechanical strain. Here, we investigated the impact of tensile strain on the gene expression profile of PDLF under normal (NS) and high salt (HS) conditions. Methods After preincubation under NS or HS (+40 & x202f;mM NaCl in medium) conditions for 24 & x202f;h, PDLF were stretched 16% for 48 & x202f;h using custom-made spherical cap silicone stamps using an established and published setup. After determination of cell number and cytotoxicity, we analyzed expression of genes involved in extracellular matrix reorganization, angiogenesis, bone remodeling, and inflammation by quantitative real-time polymerase chain reaction (RT-qPCR). Results Tensile strain did not affect the expression of genes involved in angiogenesis or extracellular matrix reorganization by PDLF, which however modulate inflammatory responses and bone remodeling in reaction to 16% static tensile strain. Salt (NaCl) treatment triggered enhanced extracellular matrix formation, expression of cyclooxygenase 2 and bone metabolism in PDLF during tensile strain. Conclusions Salt (NaCl) consumption may influence orthodontic tooth movement and periodontal bone loss via modulation of extracellular matrix and bone metabolism. Excessive salt intake during orthodontic therapy may cause adverse effects regarding periodontal inflammation and bone resorption.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ORTHODONTIC TOOTH MOVEMENT; MESSENGER-RNAS; NA+ STORAGE; CELLS; INDUCTION; MMP-8; OSTEOCLASTOGENESIS; DIFFERENTIATION; HYPERTENSION; CYTOKINES; Periodontal ligament fibroblast; Periodontal ligament (PDL); Orthodontic tooth movement; Tensile strain; Expression kinetics |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Kieferorthopädie Medicine > Lehrstuhl für Medizinische Mikrobiologie und Hygiene Medicine > Lehrstuhl für Mund-, Kiefer- und Gesichtschirurgie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 18 Mar 2021 10:04 |
| Last Modified: | 18 Mar 2021 10:04 |
| URI: | https://pred.uni-regensburg.de/id/eprint/44237 |
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