Raith, Timo and Kroeninger, Anna and Mickert, Matthias J. and Gorris, Hans H. and Matysik, Frank-Michael (2020) Enhanced resolution of generator-collector studies of enzymatic structures by means of hydrodynamic scanning electrochemical microscopy. TALANTA, 214: 120844. ISSN 0039-9140, 1873-3573
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In this report, the effects of forced convection on scanning electrochemical microscopy (SECM) studies of enzymes in the context of the generator-collector mode (G/C mode) were investigated. Forced convection was generated via an electrical high precision stirrer integrated into the electrochemical cell. Circular spots of glucose oxidase were immobilized on a gold support serving as model substrate. The diffusion layer of enzymatically generated H2O2 was characterized recording probe scan curves (PSCs) in z-direction. Furthermore, the enzyme-modified surfaces were investigated via constant-height SECM imaging in feedback mode and in G/C mode. For methodical comparison all sets of experiments were performed in quiescent solution (conventional approach) and with forced convection, respectively. In contrast to a growing diffusion layer without forced convection by applying forced convection, a constant diffusion layer of produced H2O2 was observed. Hence, via hydrodynamic SECM time-independent images within a reasonable time scale of SECM measurements in G/C mode were enabled and their resolution was enhanced.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | LIVING CELLS; ELECTRODE; GLUCOSE; VISUALIZATION; HYBRIDIZATION; MEMBRANE; SURFACES; SPOTS; Electroanalytical chemistry; Enzymes; Forced convection; Scanning electrochemical microscopy |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik > Instrumentelle Analytik (Prof. Frank-Michael Matysik) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 19 Mar 2021 13:14 |
| Last Modified: | 19 Mar 2021 13:14 |
| URI: | https://pred.uni-regensburg.de/id/eprint/44333 |
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