Schulze, Stefan and Adams, Zachary and Cerletti, Micaela and De Castro, Rosana and Ferreira-Cerca, Sebastien and Fufezan, Christian and Gimenez, Maria Ines and Hippler, Michael and Jevtic, Zivojin and Knueppel, Robert and Legerme, Georgio and Lenz, Christof and Marchfelder, Anita and Maupin-Furlow, Julie and Paggi, Roberto A. and Pfeiffer, Friedhelm and Poetsch, Ansgar and Urlaub, Henning and Pohlschroder, Mechthild (2020) The Archaeal Proteome Project advances knowledge about archaeal cell biology through comprehensive proteomics. NATURE COMMUNICATIONS, 11 (1). ISSN 2041-1723,
Full text not available from this repository. (Request a copy)Abstract
While many aspects of archaeal cell biology remain relatively unexplored, systems biology approaches like mass spectrometry (MS) based proteomics offer an opportunity for rapid advances. Unfortunately, the enormous amount of MS data generated often remains incompletely analyzed due to a lack of sophisticated bioinformatic tools and field-specific biological expertise for data interpretation. Here we present the initiation of the Archaeal Proteome Project (ArcPP), a community-based effort to comprehensively analyze archaeal proteomes. Starting with the model archaeon Haloferax volcanii, we reanalyze MS datasets from various strains and culture conditions. Optimized peptide spectrum matching, with strict control of false discovery rates, facilitates identifying>72% of the reference proteome, with a median protein sequence coverage of 51%. These analyses, together with expert knowledge in diverse aspects of cell biology, provide meaningful insights into processes such as N-terminal protein maturation, N-glycosylation, and metabolism. Altogether, ArcPP serves as an invaluable blueprint for comprehensive prokaryotic proteomics. While archaeal proteomics advanced rapidly, a comprehensive proteome database for archaea is lacking. Therefore, the authors here launch the Archaeal Proteome Project, a community-effort providing insights into archaeal cell biology via the combined reanalysis of Haloferax volcanii proteomics data.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | FALSE DISCOVERY RATES; N-TERMINAL ACETYLATION; S-LAYER GLYCOPROTEIN; HALOFERAX-VOLCANII; MASS-SPECTROMETRY; PEPTIDE IDENTIFICATION; MEMBRANE PROTEOME; STRESS RESPONSES; SIGNAL PEPTIDES; LONB PROTEASE; |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Biochemie III Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Biochemie III > Prof. Dr. Herbert Tschochner |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 22 Mar 2021 07:06 |
| Last Modified: | 22 Mar 2021 07:06 |
| URI: | https://pred.uni-regensburg.de/id/eprint/44373 |
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