Gollmer, Anita and Felgentraeger, Ariane and Maisch, Tim and Flors, Cristina (2017) Real-time imaging of photodynamic action in bacteria. JOURNAL OF BIOPHOTONICS, 10 (2). pp. 264-270. ISSN 1864-063X, 1864-0648
Full text not available from this repository. (Request a copy)Abstract
Fluorescence imaging studies of the processes leading to photodynamic inactivation of bacteria have been limited due to the small size of microorganisms as well as by the faint fluorescence of most photosensitizers. A versatile method based on highly-sensitive fluorescence microscopy is presented which allows to study, in real time, the incorporation of photosensitizers inside S. aureus upon photodynamic action. The method takes advantage of the fluorescence enhancement of phenothiazine and porphyrin photosensitizers upon entering the bacterial cytosol after the cell wall has been compromised. In combination with typical assays, such as the addition of specific enhancers of reactive oxygen species, it is possible to extract mechanistic information about the pathway of photodynamic damage at the single-cell level. Imaging experiments in deuterated buffer strongly support a Type-I mechanism for methylene blue and a very minor role of singlet oxygen.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | METHYLENE-BLUE; SINGLET OXYGEN; ESCHERICHIA-COLI; INACTIVATION; PHOTOSENSITIZER; THERAPY; PORPHYRINS; CELLS; 21ST-CENTURY; FLUORESCENCE; Fluorescence microscopy; photodynamic therapy; photosensitizers; reactive oxygen species; singlet oxygen; Staphylococcus aureus |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Dermatologie und Venerologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 14 Dec 2018 13:01 |
| Last Modified: | 11 Feb 2019 12:30 |
| URI: | https://pred.uni-regensburg.de/id/eprint/444 |
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