Nazet, Ute and Graessel, Susanne and Jantsch, Jonathan and Proff, Peter and Schroeder, Agnes and Kirschneck, Christian (2020) Early OA Stage Like Response Occurs after Dynamic Stretching of Human Synovial Fibroblasts. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 21 (11): 3874. ISSN , 1422-0067
Full text not available from this repository. (Request a copy)Abstract
As events triggering early osteoarthritis onset can be related to mechanical stress and proinflammatory signaling, we investigated the effect of different mechanical strain protocols on the expression of proinflammatory genes, as well as extracellular matrix remodelling in human synovial fibroblasts. Three distinct models of tensile stretching were applied: static isotropic tensile strain at 0 Hz, 16% tension for 48 h; short-term high-frequency cyclic tension at 1 Hz, 10% tension for 4 h; and dynamic tensile stretching for 48 h, consisting of two blocks of moderate stretching at 0.2 Hz, 2%, advanced stretching at 0.5 Hz, 15%, or a combination of both. General signs of inflammation were present after static isotropic tension, whereas short-term high-frequency cyclic tension showed increased levels of IL-6 paired with diminished levels of IL-1 beta. Reduced inflammatory effects of TNF-alpha, IL-6, and IL-1 beta were observed when exposed to advanced stretching. Long-term tensile strain induced extracellular matrix remodelling at the gene and protein levels. While hyaluronan acid synthesis was increased with static tensile strain, dynamic tensile stretching had a reducing effect. Our study revealed that proinflammatory markers were activated by mechanical strain as seen in static isotropic tension and short-term high-frequency tensile strain, whereas long-term exposure induced extracellular matrix remodelling processes.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | TUMOR-NECROSIS-FACTOR; SERUM HYALURONIC-ACID; RHEUMATOID-ARTHRITIS; GENE-EXPRESSION; ARTICULAR CHONDROCYTES; JOINT INFLAMMATION; CARTILAGE LOSS; GROWTH-FACTORS; NITRIC-OXIDE; FACTOR-ALPHA; osteoarthritis; synovial fibroblasts; mechanical strain; inflammation |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Kieferorthopädie Medicine > Lehrstuhl für Medizinische Mikrobiologie und Hygiene Medicine > Lehrstuhl für Orthopädie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 22 Mar 2021 12:39 |
| Last Modified: | 22 Mar 2021 12:39 |
| URI: | https://pred.uni-regensburg.de/id/eprint/44461 |
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