Dorn, Christoph and Schiesser, Selina and Wulkersdorfer, Beatrix and Hitzenbichler, Florian and Kees, Martin G. and Zeitlinger, Markus (2020) Determination of free clindamycin, flucloxacillin or tedizolid in plasma: Pay attention to physiological conditions when using ultrafiltration. BIOMEDICAL CHROMATOGRAPHY, 34 (6): e4820. ISSN 0269-3879, 1099-0801
Full text not available from this repository. (Request a copy)Abstract
Pharmacokinetic/pharmacodynamic indices of anti-infective drugs should be referenced to free drug concentrations. In the present study, clindamycin, flucloxacillin and tedizolid have been determined in human plasma by HPLC-UV. The drugs were separated isocratically within 3-6 min on a C-18 column using mixtures of phosphate buffer-acetonitrile of pH 7.1-7.2. Sample treatment for the determination of total drug concentrations in plasma included extraction/back-extraction (clindamycin) or protein precipitation (flucloxacillin, tedizolid). The free drug concentrations were determined after ultrafiltration. An ultrafiltration device with a membrane consisting of regenerated cellulose proved to be suitable for all drugs. Maintaining a physiological pH was crucial for clindamycin, whereas maintaining body temperature was essential for tedizolid. The methods were applied to the analysis of total and free drug concentrations in clinical samples and were sufficiently sensitive for pharmacokinetic studies and therapeutic drug monitoring.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | PROTEIN-BINDING; IMPACT; HPLC-UV; lincosamide; oxazolidinone; penicillin; protein binding; therapeutic drug monitoring |
| Subjects: | 600 Technology > 610 Medical sciences Medicine 600 Technology > 615 Pharmacy |
| Divisions: | Medicine > Lehrstuhl für Anästhesiologie Medicine > Lehrstuhl für Innere Medizin I Chemistry and Pharmacy > Institute of Pharmacy Chemistry and Pharmacy > Institute of Pharmacy > Group Clinical Pharmacy (Dr. Dorn) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 23 Mar 2021 07:33 |
| Last Modified: | 23 Mar 2021 07:33 |
| URI: | https://pred.uni-regensburg.de/id/eprint/44501 |
Actions (login required)
 |
View Item |
