Caudron-Herger, Maiwen and Pankert, Teresa and Seiler, Jeanette and Nemeth, Attila and Voit, Renate and Grummt, Ingrid and Rippe, Karsten (2015) Alu element-containing RNAs maintain nucleolar structure and function. EMBO JOURNAL, 34 (22). pp. 2758-2774. ISSN 0261-4189, 1460-2075
Full text not available from this repository. (Request a copy)Abstract
Non-coding RNAs play a key role in organizing the nucleus into functional subcompartments. By combining fluorescence microscopy and RNA deep-sequencing-based analysis, we found that RNA polymerase II transcripts originating from intronic Alu elements (aluRNAs) were enriched in the nucleolus. Antisense-oligo-mediated depletion of aluRNAs or drug-induced inhibition of RNA polymerase II activity disrupted nucleolar structure and impaired RNA polymerase I-dependent transcription of rRNA genes. In contrast, overexpression of a prototypic aluRNA sequence increased both nucleolus size and levels of pre-rRNA, suggesting a functional link between aluRNA, nucleolus integrity and pre-rRNA synthesis. Furthermore, we show that aluRNAs interact with nucleolin and target ectopic genomic loci to the nucleolus. Our study suggests an aluRNA-based mechanism that links RNA polymerase I and II activities and modulates nucleolar structure and rRNA production.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | POLYMERASE-I TRANSCRIPTION; RIBOSOMAL INTERGENIC SPACER; NONCODING RNAS; BINDING ACTIVITY; NUCLEAR-BODIES; HUMAN-CELLS; CHROMATIN; PROTEIN; GENES; COMPONENTS; Alu repeat-containing RNA; nucleolus structure and function; RNA-dependent phase separation |
| Subjects: | 500 Science > 540 Chemistry & allied sciences 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Biochemie III |
| Depositing User: | Petra Gürster |
| Date Deposited: | 26 Aug 2020 10:47 |
| Last Modified: | 26 Aug 2020 10:47 |
| URI: | https://pred.uni-regensburg.de/id/eprint/4461 |
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