Antosz, Wojciech and Deforges, Jules and Begcy, Kevin and Bruckmann, Astrid and Poirier, Yves and Dresselhaus, Thomas and Grasser, Klaus D. (2020) Critical Role of Transcript Cleavage in Arabidopsis RNA Polymerase II Transcriptional Elongation. PLANT CELL, 32 (5). pp. 1449-1463. ISSN 1040-4651, 1531-298X
Full text not available from this repository. (Request a copy)Abstract
Transcript elongation factors associate with elongating RNA polymerase II (RNAPII) to control the efficiency of mRNA synthesis and consequently modulate plant growth and development. Encountering obstacles during transcription such as nucleosomes or particular DNA sequences may cause backtracking and transcriptional arrest of RNAPII. The elongation factor TFIIS stimulates the intrinsic transcript cleavage activity of the polymerase, which is required for efficient rescue of backtracked/arrested RNAPII. A TFIIS mutant variant (TFIISmut) lacks the stimulatory activity to promote RNA cleavage, but instead efficiently inhibits unstimulated transcript cleavage by RNAPII. We could not recover viable Arabidopsis (Arabidopsis thaliana) tfIIs plants constitutively expressing TFIISmut. Induced, transient expression of TFIISmut in tfIIs plants provoked severe growth defects, transcriptomic changes and massive, transcription-related redistribution of elongating RNAPII within transcribed regions toward the transcriptional start site. The predominant site of RNAPII accumulation overlapped with the 11 nucleosome, suggesting that upon inhibition of RNA cleavage activity, RNAPII arrest prevalently occurs at this position. In the presence of TFIISmut, the amount of RNAPII was reduced, which could be reverted by inhibiting the proteasome, indicating proteasomal degradation of arrested RNAPII. Our findings suggest that polymerase backtracking/arrest frequently occurs in plant cells, and RNAPII-reactivation is essential for correct transcriptional output and proper growth/development.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | TANDEM AFFINITY PURIFICATION; FACTOR TFIIS; SEED DORMANCY; NUCLEOSOME; EXPRESSION; COMPLEX; UBIQUITYLATION; MECHANISMS; MACHINERY; PROTEINS; |
| Subjects: | 500 Science > 570 Life sciences 500 Science > 580 Botanical sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Pflanzenwissenschaften > Lehrstuhl für Zellbiologie und Pflanzenphysiologie (Prof. Dr. Klaus Grasser) Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Biochemie I > Prof. Dr. Gunter Meister |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 24 Mar 2021 11:31 |
| Last Modified: | 24 Mar 2021 11:31 |
| URI: | https://pred.uni-regensburg.de/id/eprint/44622 |
Actions (login required)
 |
View Item |
