Farka, Zdenek and Mickert, Matthias J. and Mikusova, Zuzana and Hlavacek, Antonin and Bouchalova, Pavla and Xu, Wenshu and Bouchal, Pavel and Skladal, Petr and Gorris, Hans H. (2020) Surface design of photon-upconversion nanoparticles for high-contrast immunocytochemistry. NANOSCALE, 12 (15). pp. 8303-8313. ISSN 2040-3364, 2040-3372
Full text not available from this repository. (Request a copy)Abstract
Immunohistochemistry (IHC) and immunocytochemistry (ICC) are routinely employed for the microscopic identification and diagnosis of cancerous cells in histological tissues and cell cultures. The maximally attainable contrast of conventional histological staining techniques, however, is low. While the anti-Stokes emission of photon-upconversion nanoparticles (UCNP) can efficiently eliminate optical background interference, excluding non-specific interactions of the label with the histological sample is equally important for specific immunolabeling. To address both requirements, we have designed and characterized several UCNP-based nanoconjugates as labels for the highly specific detection of the cancer biomarker HER2 on various breast cancer cell lines. An optimized streptavidin-PEG-neridronate-UCNP conjugate provided an unsurpassed signal-to-background ratio of 319, which was 50-fold better than conventional fluorescent labeling under the same experimental conditions. In combination, the absence of optical interference and non-specific binding lays the foundation for computer-based data evaluation in digital pathology.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | UPCONVERTING NANOPARTICLES; CANCER; TISSUE; CELL; |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 26 Mar 2021 11:24 |
| Last Modified: | 26 Mar 2021 11:24 |
| URI: | https://pred.uni-regensburg.de/id/eprint/44724 |
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