Farka, Zdenek and Mickert, Matthias J. and Pastucha, Matej and Mikusova, Zuzana and Skladal, Petr and Gorris, Hans H. (2020) Advances in Optical Single-Molecule Detection: En Route to Supersensitive Bioaffinity Assays. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 59 (27). pp. 10746-10773. ISSN 1433-7851, 1521-3773
Full text not available from this repository. (Request a copy)Abstract
The ability to detect low concentrations of analytes and in particular low-abundance biomarkers is of fundamental importance, e.g., for early-stage disease diagnosis. The prospect of reaching the ultimate limit of detection has driven the development of single-molecule bioaffinity assays. While many review articles have highlighted the potentials of single-molecule technologies for analytical and diagnostic applications, these technologies are not as widespread in real-world applications as one should expect. This Review provides a theoretical background on single-molecule-or better digital-assays to critically assess their potential compared to traditional analog assays. Selected examples from the literature include bioaffinity assays for the detection of biomolecules such as proteins, nucleic acids, and viruses. The structure of the Review highlights the versatility of optical single-molecule labeling techniques, including enzymatic amplification, molecular labels, and innovative nanomaterials.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | CROSS-CORRELATION SPECTROSCOPY; LINKED-IMMUNOSORBENT-ASSAY; FLUORESCENCE CORRELATION SPECTROSCOPY; ALTERNATING-LASER EXCITATION; UP-CONVERSION NANOPARTICLES; PROSTATE-SPECIFIC ANTIGEN; ULTRASENSITIVE DETECTION; QUANTUM DOTS; REAL-TIME; HOMOGENEOUS IMMUNOASSAY; digital assays; immunoassays; optical detection; signal background; single-molecule detection |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik |
| Depositing User: | Petra Gürster |
| Date Deposited: | 09 Apr 2021 08:36 |
| Last Modified: | 09 Apr 2021 08:36 |
| URI: | https://pred.uni-regensburg.de/id/eprint/44741 |
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