Omori, Marjorie Ayumi and Maranon-Vasquez, Guido Artemio and Romualdo, Priscilla Coutinho and Neto, Evandro Carneiro Martins and Stuani, Maria Bernadete Sasso and Matsumoto, Mirian Aiko Nakane and Nelson-Filho, Paulo and Proff, Peter and Leon, Jorge Esquiche and Kirschneck, Christian and Kuechler, Erika C. (2020) Effect of ovariectomy on maxilla and mandible dimensions of female rats. ORTHODONTICS & CRANIOFACIAL RESEARCH, 23 (3). pp. 342-350. ISSN 1601-6335, 1601-6343
Full text not available from this repository. (Request a copy)Abstract
Objective The role of oestrogen in craniofacial growth still remains unclear. Therefore, the present study aimed to assess the effect of oestrogen deficiency on maxilla and mandible dimensions. Setting and Sample Population The study was conducted at the Department of Pediatric Dentistry at the School of Dentistry of Ribeirao Preto, University of Sao Paulo, and used forty female Wistar rats. Material and Methods Ovariectomy (OVX) and placebo surgery (Sham) were performed when animals were twenty-one days old (prepubertal stage). Dimensions of the maxilla and mandible were assessed by craniometric analysis using radiographs, during and after puberty of the animals (45 and 63 days old, respectively). Quantitative real-time PCR and immunohistochemical analyses were performed to determine the expression and localization, respectively, of oestrogen receptor alpha (ER alpha) and oestrogen receptor beta (ER beta) in different growth sites of the evaluated structures at puberty. The differences between the groups for each outcome were evaluated using the t test with an established alpha error of 5%. Results There were significant differences between the OVX and Sham groups for horizontal and vertical linear measurements in the maxilla and the mandible at both pubertal and post-pubertal stages (P < .05). The ovariectomized rats showed significantly greater measures for all dimensions assessed. No differences in gene expression of ER alpha and ER beta were identified at the different growth sites between the OVX and Sham groups (P > .05). Immunohistochemical analyses revealed the presence of both oestrogen receptors in osteoblasts and chondrocytes in the midpalatal suture and mandibular condyle, respectively, in the OVX and Sham groups. Conclusion Our results suggest that oestrogen deficiency from the prepubertal stage might increase the growth of the maxilla and mandible in female rats.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ESTROGEN-RECEPTOR-ALPHA; SEX-HORMONE DISTURBANCES; GROWTH-PLATE; AROMATASE DEFICIENCY; GENE-EXPRESSION; BONE-GROWTH; ESTRADIOL; MECHANISMS; CHILDHOOD; ANDROGEN; mandible; maxilla; oestrogen receptor alpha; oestrogen receptor beta; oestrogens |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Kieferorthopädie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 26 Mar 2021 12:36 |
| Last Modified: | 26 Mar 2021 12:36 |
| URI: | https://pred.uni-regensburg.de/id/eprint/44747 |
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