Grevenstein, David and Mamilos, Andreas and Schmitt, Volker H. and Niedermair, Tanja and Wagner, Willi and Kirkpatrick, C. James and Brochhausen, Christoph (2021) Excellent histological results in terms of articular cartilage regeneration after spheroid-based autologous chondrocyte implantation (ACI). KNEE SURGERY SPORTS TRAUMATOLOGY ARTHROSCOPY, 29. pp. 417-421. ISSN 0942-2056, 1433-7347
Full text not available from this repository. (Request a copy)Abstract
Purpose Traumatic lesions of articular cartilage represent a crucial risk factor for osteoarthritis. Even if several strategies exist to treat such damages, the optimal solution has not yet been found. A new strategy represents the scaffold-free spheroid-based autologous chondrocyte transplantation. In this method, spheroids of chondrocytes are synthesized after chondrocyte isolation and expansion, followed by the implantation in a second intervention. Methods Fine Jamshidi-needle biopsies from five patients (one from each patient, o 2 mm) treated with a spheroid-based autologous chondrocyte implantation (ACI) after traumatic lesions of the articular cartilage of the knee were analysed histologically and immunohistologically for collagen II, collagen X and aggrecan expression. The indication for a second look arthroscopy was given by arthrofibrosis or meniscus-lesions, respectively. The time between ACI and second-look arthroscopy ranged between 6 and 16 months. Results In all patients, the histological examinations revealed an avascular cartilage tissue with a homogenic extracellular matrix. The subchondral bone neither showed bleeding, necrosis nor hypertrophy. A homogenous alcian blue staining indicated high amounts of mucopolysaccharides and glycosaminoglycans. Collagen II staining was highly positive, whereas collagen X staining was negative in every patient, ruling out hypertrophic chondrocyte differentiation. In addition, intense aggrecan staining indicated a strong expression of this extracellular matrix component. Conclusion The present case series represents the first histological and immunohistological analyses of spheroid-based ACI in humans. Spheroid-based ACI revealed excellent histological results regarding the regeneration of hyaline articular cartilage. These results indicate that spheroid based ACI is a promising strategy for treating traumatic lesions of the articular cartilage of the knee.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | OSTEOCHONDRAL DEFECTS; KNEE; PERIOSTEUM; REPAIR; Cartilage; Autologous chondrocyte transplantation; Chondrocytes; Spheroids; MACI |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Orthopädie Medicine > Lehrstuhl für Pathologie |
| Depositing User: | Petra Gürster |
| Date Deposited: | 08 Apr 2021 06:50 |
| Last Modified: | 08 Apr 2021 06:50 |
| URI: | https://pred.uni-regensburg.de/id/eprint/44751 |
Actions (login required)
 |
View Item |
