Hofmann, Carola and Kaiser, Barbara and Maerkl, Susanne and Duerkop, Axel and Baeumner, Antje J. (2020) Cationic liposomes for generic signal amplification strategies in bioassays. ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 412 (14). pp. 3383-3393. ISSN 1618-2642, 1618-2650
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Liposomes have been widely applied in bioanalytical assays. Most liposomes used bare negative charges to prevent non-specific binding and increase colloidal stability. Here, in contrast, highly stable, positively charged liposomes entrapping the fluorescent dye sulforhodamine B (SRB) were developed to serve as a secondary, non-specific label, and signal amplification tool in bioanalytical systems by exploiting their electrostatic interaction with negatively charged vesicles, surfaces, and microorganisms. The cationic liposomes were optimized for long-term stability (> 5 months) and high dye entrapment yield. Their capability as secondary, non-specific labels was first successfully proven through electrostatic interactions of cationic and anionic liposomes using dynamic light scattering, and then in a bioassay with fluorescence detection leading to an enhancement factor of 8.5 without any additional surface blocking steps. Moreover, the cationic liposomes bound efficiently to anionic magnetic beads were stable throughout magnetic separation procedures and could hence serve directly as labels in magnetic separation and purification strategies. Finally, the electrostatic interaction was exploited for the direct, simple, non-specific labeling of gram-negative bacteria. Isolated Escherichia coli cells were chosen as models and direct detection was demonstrated via fluorescent and chemiluminescent liposomes. Thus, these cationic liposomes can be used as generic labels for the development of ultrasensitive bioassays based on electrostatic interaction without the need for additional expensive recognition units like antibodies, where desired specificity is already afforded through other strategies.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | CHOLESTEROL; VESICLES; LIPIDS; LABEL; Liposomes; Electrostatic interaction; Bioanalysis; Bacteria; E. coli |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik > Chemo- und Biosensorik (Prof. Antje J. Bäumner, formerly Prof. Wolfbeis) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 26 Mar 2021 13:17 |
| Last Modified: | 26 Mar 2021 13:17 |
| URI: | https://pred.uni-regensburg.de/id/eprint/44765 |
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