Kiel, Christina and Berber, Patricia and Karlstetter, Marcus and Aslanidis, Alexander and Strunz, Tobias and Langmann, Thomas and Grassmann, Felix and Weber, Bernhard H. F. (2020) A Circulating MicroRNA Profile in a Laser-Induced Mouse Model of Choroidal Neovascularization. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 21 (8): 2689. ISSN , 1422-0067
Full text not available from this repository. (Request a copy)Abstract
Choroidal neovascularization (CNV) is a pathological process in which aberrant blood vessels invade the subretinal space of the mammalian eye. It is a characteristic feature of the prevalent neovascular age-related macular degeneration (nAMD). Circulating microRNAs (cmiRNAs) are regarded as potentially valuable biomarkers for various age-related diseases, including nAMD. Here, we investigated cmiRNA expression in an established laser-induced CNV mouse model. Upon CNV induction in C57Bl/6 mice, blood-derived cmiRNAs were initially determined globally by RNA next generation sequencing, and the most strongly dysregulated cmiRNAs were independently replicated by quantitative reverse transcription PCR (RT-qPCR) in blood, retinal, and retinal pigment epithelium (RPE)/choroidal tissue. Our findings suggest that two miRNAs, mmu-mir-486a-5p and mmur-mir-92a-3p, are consistently dysregulated during CNV formation. Furthermore, in functional in vitro assays, a significant impact of mmu-mir-486a-5p and mmu-mir-92a-3p on murine microglial cell viability was observed, while mmu-mir-92a-3p also showed an impact on microglial mobility. Taken together, we report a robust dysregulation of two miRNAs in blood and RPE/choroid after laser-induced initiation of CNV lesions in mice, highlighting their potential role in pathology and eventual therapy of CNV-associated complications.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ENDOTHELIAL GROWTH-FACTOR; RETINAL-PIGMENT EPITHELIUM; MACULAR DEGENERATION; POTENTIAL BIOMARKERS; NATURAL-HISTORY; IN-VITRO; EXPRESSION; CELLS; ANGIOGENESIS; GENETICS; cmiRNA regulation; age-related macular degeneration; laser-induced choroidal neovascularization; biomarker |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Humangenetik |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 29 Mar 2021 04:23 |
| Last Modified: | 29 Mar 2021 04:23 |
| URI: | https://pred.uni-regensburg.de/id/eprint/44789 |
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