Ritschl, Paul and Hofhansel, Lena and Floerchinger, Bernhard and Oberhuber, Rupert and Oellinger, Robert and Pratschke, Johann and Kotsch, Katja (2020) Brain Death Induction in Mice Using Intra-Arterial Blood Pressure Monitoring and Ventilation via Tracheostomy. JOVE-JOURNAL OF VISUALIZED EXPERIMENTS (158): e60831. ISSN 1940-087X,
Full text not available from this repository. (Request a copy)Abstract
While both living donation and donation after circulatory death provide alternative opportunities for organ transplantation, donation after donor brain death (BD) still represents the major source for solid transplants. Unfortunately, the irreversible loss of brain function is known to induce multiple pathophysiological changes, including hemodynamic as well as hormonal modifications, finally leading to a systemic inflammatory response. Models that allow a systematic investigation of these effects in vivo are scarce. We present a murine model of BD induction, which could aid investigations into the devastating effects of BD on allograft quality. After implementing intra-arterial blood pressure measurement via the common carotid artery and reliable ventilation via a tracheostomy, BD is induced by steadily increasing intracranial pressure using a balloon catheter. Four hours after BD induction, organs may be harvested for analysis or for further transplantation procedures. Our strategy enables the comprehensive analysis of donor BD in a murine model, therefore allowing an in-depth understanding of BD-related effects in solid organ transplantation and potentially paving the way to optimized organ preconditioning.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | MODEL; ACTIVATION; INJURY; ORGANS; Neuroscience; Issue 158; transplantation; brain death; organ donation; murine; blood pressure measurement; tracheostomy |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Herz-, Thorax- und herznahe Gefäßchirurgie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 29 Mar 2021 05:12 |
| Last Modified: | 29 Mar 2021 05:12 |
| URI: | https://pred.uni-regensburg.de/id/eprint/44803 |
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