Shoemark, Amelia and Boon, Mieke and Brochhausen, Christoph and Bukowy-Bieryllo, Zuzanna and De Santi, Maria M. and Goggin, Patricia and Griffin, Paul and Hegele, Richard G. and Hirst, Robert A. and Leigh, Margaret W. and Lupton, Alison and MacKenney, Karen and Omran, Heymut and Pache, Jean-Claude and Pinto, Andreia and Reinholt, Finn P. and Schroeder, Josep and Yiallouros, Panayotis and Escudier, Estelle (2020) International consensus guideline for reporting transmission electron microscopy results in the diagnosis of primary ciliary dyskinesia (BEAT PCD TEM Criteria). EUROPEAN RESPIRATORY JOURNAL, 55 (4): 1900725. ISSN 0903-1936, 1399-3003
Full text not available from this repository. (Request a copy)Abstract
Primary ciliary dyskinesia (PCD) is a heterogeneous genetic condition. European and North American diagnostic guidelines recommend transmission electron microscopy (TEM) as one of a combination of tests to confirm a diagnosis. However, there is no definition of what constitutes a defect or consensus on reporting terminology. The aim of this project was to provide an internationally agreed ultrastructural classification for PCD diagnosis by TEM. A consensus guideline was developed by PCD electron microscopy experts representing 18 centres in 14 countries. An initial meeting and discussion were followed by a Delphi consensus process. The agreed guideline was then tested, modified and retested through exchange of samples and electron micrographs between the 18 diagnostic centres. The final guideline a) provides agreed terminology and a definition of Class 1 defects which are diagnostic for PCD; b) identifies Class 2 defects which can indicate a diagnosis of PCD in combination with other supporting evidence; c) describes features which should be included in a ciliary ultrastructure report to assist multidisciplinary diagnosis of PCD; and d) defines adequacy of a diagnostic sample. This tested and externally validated statement provides a clear guideline for the diagnosis of PCD by TEM which can be used to standardise diagnosis internationally.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | WORKING CLASSIFICATION; MUTATIONS; DEFECTS; IMMUNOFLUORESCENCE; PREVALENCE; GENERATION; SECONDARY; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Pathologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 29 Mar 2021 05:15 |
| Last Modified: | 29 Mar 2021 05:15 |
| URI: | https://pred.uni-regensburg.de/id/eprint/44805 |
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