Wassmann, Torsten and Schubert, Andrea and Malinski, Felix and Rosentritt, Martin and Krohn, Sebastian and Techmer, Kirsten and Buergers, Ralf (2020) The antimicrobial and cytotoxic effects of a copper-loaded zinc oxide phosphate cement. CLINICAL ORAL INVESTIGATIONS, 24 (11). pp. 3899-3909. ISSN 1432-6981, 1436-3771
Full text not available from this repository. (Request a copy)Abstract
Objectives Evidence about modifications of dental luting materials to minimize biological failure at the "marginal gap" between teeth and fixed prosthodontics is scarce. We compared a copper-modified (Co-ZOP) and a conventional zinc oxide phosphate cement (ZOP) in terms of antimicrobial and cytotoxic potentials in vitro and in vivo. Materials and methods Specimens of ZOP and Co-ZOP were characterized by the mean arithmetic roughness (Ra) and surface free energy (SFE). Powder components were examined using scanning electron microscopy (SEM). Energy-dispersive X-ray spectroscopy (EDX) showed elemental material compositions. In vitro microbial adhesion was shown using SEM, luminescence, and fluorescence assays. CCK-8 assays of mouse fibroblasts (L929) and human gingival fibroblasts (GF-1) were performed after 6, 24, and 48 h of specimen incubation. In vivo, ZOP and Co-ZOP specimens were applied intraorally for 12 h; biofilm accumulation was shown using SEM. Results Ra of ZOP and Co-ZOP showed no significant differences; SFE was significantly higher for Co-ZOP. EDX exhibited minor copper radiation for Co-ZOP, none for ZOP. In vitro fungal adhesion to Co-ZOP was significantly higher than to ZOP; in vitro streptococcal adhesion, cytotoxicity, and in vivo biofilm formation were not significantly different. Conclusions Co-ZOP showed low surface allocations of copper with no improved antimicrobial properties compared with conventional ZOP in vitro or in vivo.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | SURFACE-ROUGHNESS; CANDIDA-ALBICANS; BACTERIAL ADHESION; DENTAL CEMENTS; IN-VIVO; MICROBIAL COMMUNITIES; BIOFILM FORMATION; TOXICITY; NANOPARTICLES; ANTIBACTERIAL; Dental luting material; Zinc oxide phosphate cement; Copper; Candida albicans; Streptococcus sanguinis; Biofilm; Cytotoxicity |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Zahnärztliche Prothetik |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 29 Mar 2021 07:13 |
| Last Modified: | 29 Mar 2021 07:13 |
| URI: | https://pred.uni-regensburg.de/id/eprint/44909 |
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