Fabritius, Martin and Al-Munajjed, Amir Andreas and Freytag, Christiane and Juelke, Henriette and Zehe, Markus and Lemarchand, Thomas and Arts, Jacobus J. and Schumann, Detlef and Alt, Volker and Sternberg, Katrin (2020) Antimicrobial Silver Multilayer Coating for Prevention of Bacterial Colonization of Orthopedic Implants. MATERIALS, 13 (6): 1415. ISSN , 1996-1944
Full text not available from this repository. (Request a copy)Abstract
Due to increasing rates of periprosthetic joint infections (PJI), new approaches are needed to minimize the infection risk. The first goal of this study was to modify a well-established infection model to test surface-active antimicrobial systems. The second goal was to evaluate the antimicrobial activity of a silver multilayer (SML) coating. In vitro tests with SML items showed a >4 Log reduction in a proliferation assay and a 2.2 Log reduction in an agar immersion test (7 d). In the in vivo model blank and SML coated K-wires were seeded with similar to 2 x 10(4) CFU of a methicillin-sensitive Staphylococcus epidermidis (MSSE) and inserted into the intramedullary tibial canal of rabbits. After 7 days, the animals were sacrificed and a clinical, microbiological and histological analysis was performed. Microbiology showed a 1.6 Log pathogen reduction on the surface of SML items (p = 0.022) and in loosely attached tissue (p = 0.012). In the SML group 7 of 12 SML items were completely free of pathogens (cure rate = 58%, p = 0.002), while only 1 of 12 blank items were free of pathogens (cure rate = 8%, p = 0.110). No silver was detected in the blood or urine of the SML treated animals and only scarcely in the liver or adjacent lymph nodes. In summary, an in vivo infection model to test implants with bacterial pre-incubation was established and the antimicrobial activity of the SML coating was successfully proven.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | COATED MEGAPROSTHESES; PERIPROSTHETIC INFECTION; BONE TISSUE; ENDOPROSTHESES; CONTAMINATION; REDUCTION; TRAUMA; TUMOR; IONS; periprosthetic joint infections; infection prophylaxis; Staphylococcus epidermidis; in vivo osteomyelitis model |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Unfallchirurgie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 29 Mar 2021 09:33 |
| Last Modified: | 29 Mar 2021 09:33 |
| URI: | https://pred.uni-regensburg.de/id/eprint/44967 |
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