Wagner, Florian and Ott, Tankred and Schall, Maximilian and Lautenschlager, Ulrich and Vogt, Robert and Oberprieler, Christoph (2020) Taming the Red Bastards: Hybridisation and species delimitation in the Rhodanthemum arundanum-group (Compositae, Anthemideae). MOLECULAR PHYLOGENETICS AND EVOLUTION, 144: 106702. ISSN 1055-7903, 1095-9513
Full text not available from this repository. (Request a copy)Abstract
Delineating species boundaries in a group of recently diverged lineages is challenging due to minor morphological differences, low genetic differentiation and the occurrence of gene flow among taxa. Here, we employ traditional Sanger sequencing and restriction-site associated DNA (RAD) sequencing, to investigate species delimitation in the close-knit Moroccan daisy group around Rhodanthemum arundanum B.H.Wilcox & al. that diverged recently during the Quaternary. After evaluation of genotyping errors and parameter optimisation in the course of de-novo assembly of RADseq reads in IPYRAD, we assess hybridisation patterns in the study group based On different data assemblies and methods (NEIGHBOR-NET networks, FASTSTRUCTURE and ABBA-BABA tests). RADseq data and Sanger sequences are subsequently used for delimitation of species, using both, multi-species coalescent methods (STACEY and SNAPP) and a novel approach based on consensus k-means clustering. In addition to the unveiling of two novel subspecies in the R. anindanum-group, our study provides insights into the performance of different species delimitation methods in the presence of hybridisation and varying quantities of data.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | PHYLOGENETIC-RELATIONSHIPS; RETICULATE RELATIONSHIPS; CHROMOSOME-NUMBERS; LEUCANTHEMUM MILL.; NONCODING REGIONS; CLASS DISCOVERY; DNA-SEQUENCES; GENE TREES; R-PACKAGE; DE-NOVO; Consensus k-means clustering; RADseq; Hybridisation; IPYRAD; Parameter optimisation; Species delimitation |
| Subjects: | 500 Science > 580 Botanical sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Pflanzenwissenschaften > Group Plant Systematics and Evolution (Prof. Dr. Christoph Oberprieler) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 30 Mar 2021 08:08 |
| Last Modified: | 30 Mar 2021 08:08 |
| URI: | https://pred.uni-regensburg.de/id/eprint/45053 |
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