Haehnel, Viola and Dormann, Frauke and Nitsopoulos, Athanasios and Friedle, Albrecht and Ahrens, Norbert (2017) A method for the quantification of 8-methoxypsoralen by mass spectrometry for offline extracorporeal photopheresis. PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES, 16 (2). pp. 193-200. ISSN 1474-905X, 1474-9092
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Background: Extracorporeal photopheresis (ECP) is an efficient method to treat various autoimmune diseases, cutaneous T-cell lymphoma, and graft-versus-host disease. It is based on the ex vivo inactivation of lymphocytes by 8-methoxypsoralen (8-MOP)/UV light treatment. Despite the adhesive, lipophilic nature of 8-MOP, no quality control is established for the ECP procedure. Methods: We developed a sensitive high-performance liquid chromatography/tandem mass spectrometry (HPLC-MS/MS) assay to monitor residual 8-MOP concentration after UVA irradiation in the whole blood supernatant after acetonitrile precipitation. Results: The preanalytical stability of 8-MOP exceeded 7 days, allowing batch mode analysis. Linearity was determined with R-2 above 0.99. The 8-MOP concentrations decreased exponentially after UV exposure, with decay constants of 0.0259 in plasma and 0.0528 in saline. The recovery of 8-MOP in photopheresates was about 68%, indicating binding to DNA as well as to plastic structures. UVA induced no 8-MOP fragmentation, but caused self-adducts under extreme conditions (10-fold UV dosage). Conclusions: Detection of 8-MOP proved to be feasible and demonstrated that the doses were in the pharmaceutically active range.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | PHOTOCHEMOTHERAPY; PHARMACOKINETICS; PHOTOCHEMISTRY; CHROMATOGRAPHY; PROLIFERATION; PSORALENS; KINETICS; CELLS; UVA; MS; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Klinische Chemie und Laboratoriumsmedizin |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 14 Dec 2018 13:01 |
| Last Modified: | 26 Feb 2019 07:24 |
| URI: | https://pred.uni-regensburg.de/id/eprint/451 |
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