Sparks, David S. and Saifzadeh, Siamak and Savi, Flavia Medeiros and Dlaska, Constantin E. and Berner, Arne and Henkel, Jan and Reichert, Johannes C. and Wullschleger, Martin and Ren, Jiongyu and Cipitria, Amaia and McGovern, Jacqui A. and Steck, Roland and Wagels, Michael and Woodruff, Maria Ann and Schuetz, Michael A. and Hutmacher, Dietmar W. (2020) A preclinical large-animal model for the assessment of critical-size load-bearing bone defect reconstruction. NATURE PROTOCOLS, 15 (3). pp. 877-924. ISSN 1754-2189, 1750-2799
Full text not available from this repository. (Request a copy)Abstract
This protocol describes how to establish an ovine critical-size, segmental bone defect model to study bone regeneration and reconstruction. Critical-size bone defects, which require large-volume tissue reconstruction, remain a clinical challenge. Bone engineering has the potential to provide new treatment concepts, yet clinical translation requires anatomically and physiologically relevant preclinical models. The ovine critical-size long-bone defect model has been validated in numerous studies as a preclinical tool for evaluating both conventional and novel bone-engineering concepts. With sufficient training and experience in large-animal studies, it is a technically feasible procedure with a high level of reproducibility when appropriate preoperative and postoperative management protocols are followed. The model can be established by following a procedure that includes the following stages: (i) preoperative planning and preparation, (ii) the surgical approach, (iii) postoperative management, and (iv) postmortem analysis. Using this model, full results for peer-reviewed publication can be attained within 2 years. In this protocol, we comprehensively describe how to establish proficiency using the preclinical model for the evaluation of a range of bone defect reconstruction options.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | OVINE MODEL; IN-VIVO; SCAFFOLD ARCHITECTURE; INTRAMEDULLARY NAIL; SEGMENTAL DEFECT; SHEEP; REGENERATION; PHARMACOKINETICS; IMPLANTATION; DELIVERY; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Unfallchirurgie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 30 Mar 2021 08:29 |
| Last Modified: | 30 Mar 2021 08:29 |
| URI: | https://pred.uni-regensburg.de/id/eprint/45111 |
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