Kreitner, Raphael and Munte, Claudia E. and Singer, Katrin and Stetter, Karl O. and Horn, Gudrun and Kremer, Werner and Kalbitzer, Hans Robert (2020) Complete sequential assignment and secondary structure prediction of the cannulae forming protein CanA from the hyperthermophilic archaeon Pyrodictium abyssi. BIOMOLECULAR NMR ASSIGNMENTS, 14 (1). pp. 141-146. ISSN 1874-2718, 1874-270X
Full text not available from this repository. (Request a copy)Abstract
CanA from Pyrodictium abyssi forms a heat-resistant organic hollow-fiber network together with CanB and CanC. An N-terminally truncated construct of CanA (K-1-CanA) gave NMR spectra of good quality that could be assigned by three-dimensional NMR methods on N-15 and C-13-N-15 enriched protein. We assigned the chemical shifts of 96% of all backbone H-1(N) atoms, 98% of all backbone N-15 atoms, 100% of all C-13(alpha) atoms, 100% of all H-1(alpha) atoms, 90% of all C-13 ' atoms, and 100% of the C-13(beta) atoms. Two short helices and 10 beta-strands are estimated from an analysis of the chemical shifts leading to a secondary structure content of K-1-CanA of 6% helices, 44% beta-pleated sheets, and 50% coils.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | NOV; Pyrodictium abyssi; CanA; Cannulae; Extremophile; Solution NMR; Assignments |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biophysik und physikalische Biochemie > Prof. Dr. Dr. Hans Robert Kalbitzer Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Mikrobiologie (Archaeenzentrum) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 30 Mar 2021 10:23 |
| Last Modified: | 30 Mar 2021 10:23 |
| URI: | https://pred.uni-regensburg.de/id/eprint/45116 |
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