Bedal, Konstanze B. and Graessel, Susanne and Spanier, Gerrit and Reichert, Torsten E. and Bauer, Richard J. (2015) The NC11 domain of human collagen XVI induces vasculogenic mimicry in oral squamous cell carcinoma cells. CARCINOGENESIS, 36 (11). pp. 1429-1439. ISSN 0143-3334, 1460-2180
Full text not available from this repository. (Request a copy)Abstract
Collagen XVI, a fibril-associated collagen with interrupted triple helix (FACIT) collagen, is involved in oral squamous cell carcinoma (OSCC) and glioblastoma progression. The NC11 domain of collagen XVI has been described previously with a strong implication in physiological processes. We detected the non-collagenous (NC) 11-domain in supernatants of OSCC cells after recombinant expression of full-length collagen XVI and in sera from OSCC patients and healthy individuals. Stable expression of NC11-green fluorescent protein (GFP) fusion protein in OSCC cells initiated proliferation control and block of anchorage-independent growth. Moreover, the NC11 domain triggered the generation of tubular-like net structures on laminin-rich matrix in contrast to mock-GFP control cells and cells expressing full-length collagen XVI. Taqman r quantitative PCR and diaminobenzidine staining in 2D- and 3D cell culture revealed a significantly increased gene and protein expression of VEGFR1, VEGFR2 and uPAR in recombinant NC11-GFP-expressing cells. Specific VEGF receptor inhibition with Axitinib or fetal calf serum heat inactivation prevented formation of tubular-like net structures. Accordantly, NC11-GFP coated culture slides led to an increase of focal adhesion contact formation and the upregulation of VEGFR1 and uPAR in three different non-transfected OSCC cell lines. In summary, we suggest that the NC11 domain of collagen XVI is a potential biomarker for OSCC and triggers vasculogenic mimicry via upregulation of endothelial receptors VEGFR1, VEGFR2 and uPAR in 2D- and 3D OSCC cell culture conditions.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | AGGRESSIVE MELANOMA PHENOTYPE; ANGIOGENESIS IN-VITRO; UROKINASE RECEPTOR; ENDOTHELIAL-CELLS; TUMOR-GROWTH; P-CADHERIN; EXPRESSION; PROTEIN; CANCER; PROLIFERATION; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Mund-, Kiefer- und Gesichtschirurgie Medicine > Lehrstuhl für Orthopädie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 06 May 2019 08:38 |
| Last Modified: | 06 May 2019 08:38 |
| URI: | https://pred.uni-regensburg.de/id/eprint/4514 |
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