Engelmann, S. and Ruewe, M. and Geis, S. and Taeger, C. D. and Kehrer, M. and Tamm, E. R. and Bleys, R. L. A. W. and Zeman, F. and Prantl, L. and Kehrer, A. (2020) Rapid and Precise Semi-Automatic Axon Quantification in Human Peripheral Nerves. SCIENTIFIC REPORTS, 10 (1): 1935. ISSN 2045-2322,
Full text not available from this repository. (Request a copy)Abstract
We developed a time-efficient semi-automated axon quantification method using freeware in human cranial nerve sections stained with paraphenylenediamine (PPD). It was used to analyze a total of 1238 facial and masseteric nerve biopsies. The technique was validated by comparing manual and semi-automated quantification of 129 (10.4%) randomly selected biopsies. The software-based method demonstrated a sensitivity of 94% and a specificity of 87%. Semi-automatic axon counting was significantly faster (p<0.001) than manual counting. It took 1hour and 47minutes for all 129 biopsies (averaging 50sec per biopsy, 0.04seconds per axon). The counting process is automatic and does not need to be supervised. Manual counting took 21hours and 6minutes in total (average 9minutes and 49seconds per biopsy, 0.52seconds per axon). Our method showed a linear correlation to the manual counts (R=0.944 Spearman rho). Attempts have been made by several research groups to automate axonal load quantification. These methods often require specific hard- and software and are therefore only accessible to a few specialized laboratories. Our semi-automated axon quantification is precise, reliable and time-sparing using publicly available software and should be useful for an effective axon quantification in various human peripheral nerves.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | SKELETAL-MUSCLE REINNERVATION; SPINAL ACCESSORY NERVE; FACIAL-NERVE; MORPHOMETRIC-ANALYSIS; IMAGE-ANALYSIS; LOAD; GRAFT; REGENERATION; PALSY; |
| Subjects: | 500 Science > 570 Life sciences 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Zentren des Universitätsklinikums Regensburg > Zentrum für Klinische Studien Medicine > Zentren des Universitätsklinikums Regensburg > Zentrum für Plastische-, Hand- und Wiederherstellungschirurgie Biology, Preclinical Medicine > Institut für Anatomie > Lehrstuhl für Humananatomie und Embryologie > Prof. Dr. Ernst Tamm |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 30 Mar 2021 09:41 |
| Last Modified: | 30 Mar 2021 09:41 |
| URI: | https://pred.uni-regensburg.de/id/eprint/45142 |
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