Sorensen, Brian B. and Ehrnsberger, Hans F. and Esposito, Silvia and Pfab, Alexander and Bruckmann, Astrid and Hauptmann, Judith and Meister, Gunter and Merkl, Rainer and Schubert, Thomas and Laengst, Gernot and Melzer, Michael and Grasser, Marion and Grasser, Klaus D. (2017) The Arabidopsis THO/TREX component TEX1 functionally interacts with MOS11 and modulates mRNA export and alternative splicing events. PLANT MOLECULAR BIOLOGY, 93 (3). pp. 283-298. ISSN 0167-4412, 1573-5028
Full text not available from this repository. (Request a copy)Abstract
We identify proteins that associate with the THO core complex, and show that the TEX1 and MOS11 components functionally interact, affecting mRNA export and splicing as well as plant development. TREX (TRanscription-EXport) is a multiprotein complex that plays a central role in the coordination of synthesis, processing and nuclear export of mRNAs. Using targeted proteomics, we identified proteins that associate with the THO core complex of Arabidopsis TREX. In addition to the RNA helicase UAP56 and the mRNA export factors ALY2-4 and MOS11 we detected interactions with the mRNA export complex TREX-2 and multiple spliceosomal components. Plants defective in the THO component TEX1 or in the mRNA export factor MOS11 (orthologue of human CIP29) are mildly affected. However, tex1 mos11 double-mutant plants show marked defects in vegetative and reproductive development. In tex1 plants, the levels of tasiRNAs are reduced, while miR173 levels are decreased in mos11 mutants. In nuclei of mos11 cells increased mRNA accumulation was observed, while no mRNA export defect was detected with tex1 cells. Nevertheless, in tex1 mos11 double-mutants, the mRNA export defect was clearly enhanced relative to mos11. The subnuclear distribution of TEX1 substantially overlaps with that of splicing-related SR proteins and in tex1 plants the ratio of certain alternative splicing events is altered. Our results demonstrate that Arabidopsis TEX1 and MOS11 are involved in distinct steps of the biogenesis of mRNAs and small RNAs, and that they interact regarding some aspects, but act independently in others.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | HUMAN TREX COMPLEX; PROTEOMIC ANALYSIS; GENE-EXPRESSION; BOX PROTEIN; THO COMPLEX; TRANSCRIPTION ELONGATION; MRNP BIOGENESIS; NUCLEAR EXPORT; MACHINERY; GROWTH; mRNA processing/export; Chromatin; Protein complexes; RNA polymerase II; Development |
| Subjects: | 500 Science > 580 Botanical sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Pflanzenwissenschaften > Lehrstuhl für Zellbiologie und Pflanzenphysiologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 14 Dec 2018 13:01 |
| Last Modified: | 14 Feb 2019 16:05 |
| URI: | https://pred.uni-regensburg.de/id/eprint/453 |
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