Rendeiro, Andre F. and Krausgruber, Thomas and Fortelny, Nikolaus and Zhao, Fangwen and Penz, Thomas and Farlik, Matthias and Schuster, Linda C. and Nemc, Amelie and Tasnady, Szabolcs and Reti, Marienn and Matrai, Zoltan and Alpar, Donat and Boedoer, Csaba and Schmidl, Christian and Bock, Christoph (2020) Chromatin mapping and single-cell immune profiling define the temporal dynamics of ibrutinib response in CLL. NATURE COMMUNICATIONS, 11 (1): 577. ISSN 2041-1723,
Full text not available from this repository. (Request a copy)Abstract
The Bruton tyrosine kinase (BTK) inhibitor ibrutinib provides effective treatment for patients with chronic lymphocytic leukemia (CLL), despite extensive heterogeneity in this disease. To define the underlining regulatory dynamics, we analyze high-resolution time courses of ibrutinib treatment in patients with CLL, combining immune-phenotyping, single-cell transcriptome profiling, and chromatin mapping. We identify a consistent regulatory program starting with a sharp decrease of NF-kappa B binding in CLL cells, which is followed by reduced activity of lineage-defining transcription factors, erosion of CLL cell identity, and acquisition of a quiescence-like gene signature. We observe patient-to-patient variation in the speed of execution of this program, which we exploit to predict patient-specific dynamics in the response to ibrutinib based on the pre-treatment patient samples. In aggregate, our study describes time-dependent cellular, molecular, and regulatory effects for therapeutic inhibition of B cell receptor signaling in CLL, and it establishes a broadly applicable method for epigenome/transcriptome-based treatment monitoring.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | CHRONIC LYMPHOCYTIC-LEUKEMIA; NF-KAPPA-B; GENE-EXPRESSION; T-CELL; RECURRENT MUTATIONS; BINDING PROTEINS; DNA METHYLATION; INHIBITOR; PHENOTYPE; ALIGNMENT; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Zentren des Universitätsklinikums Regensburg > Regensburger Centrum für Interventionelle Immunologie (RCI) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 09 Apr 2021 08:11 |
| Last Modified: | 09 Apr 2021 08:11 |
| URI: | https://pred.uni-regensburg.de/id/eprint/45302 |
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