Minderjahn, Julia and Schmidt, Andreas and Fuchs, Andreas and Schill, Rudolf and Raithel, Johanna and Babina, Magda and Schmidl, Christian and Gebhard, Claudia and Schmidhofer, Sandra and Mendes, Karina and Ratermann, Anna and Glatz, Dagmar and Nuetzel, Margit and Edinger, Matthias and Hoffman, Petra and Spang, Rainer and Laengst, Gernot and Imhof, Axel and Rehli, Michael (2020) Mechanisms governing the pioneering and redistribution capabilities of the non-classical pioneer PU.1. NATURE COMMUNICATIONS, 11 (1): 402. ISSN 2041-1723,
Full text not available from this repository. (Request a copy)Abstract
Establishing gene regulatory networks during differentiation or reprogramming requires master or pioneer transcription factors (TFs) such as PU.1, a prototype master TF of hematopoietic lineage differentiation. To systematically determine molecular features that control its activity, here we analyze DNA-binding in vitro and genome-wide in vivo across different cell types with native or ectopic PU.1 expression. Although PU.1, in contrast to classical pioneer factors, is unable to access nucleosomal target sites in vitro, ectopic induction of PU.1 leads to the extensive remodeling of chromatin and redistribution of partner TFs. De novo chromatin access, stable binding, and redistribution of partner TFs both require PU.1's N-terminal acidic activation domain and its ability to recruit SWI/SNF remodeling complexes, suggesting that the latter may collect and distribute co-associated TFs in conjunction with the non-classical pioneer TF PU.1.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | TRANSCRIPTION FACTORS; SWI/SNF COMPLEX; GENE-EXPRESSION; REGULATORY ELEMENTS; ACTIVATION DOMAIN; MACROPHAGE; DIFFERENTIATION; REPRESSES; ENHANCERS; SELECTION; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin III (Hämatologie und Internistische Onkologie) Medicine > Zentren des Universitätsklinikums Regensburg > Regensburger Centrum für Interventionelle Immunologie (RCI) Medicine > Institut für Funktionelle Genomik > Lehrstuhl für Statistische Bioinformatik (Prof. Spang) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 01 Apr 2021 10:35 |
| Last Modified: | 01 Apr 2021 10:35 |
| URI: | https://pred.uni-regensburg.de/id/eprint/45334 |
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